Extracellular vesicles as possible plasma markers and mediators in patients with sepsis-associated delirium

• Verfasst von: Plaschke, Konstanze [VerfasserIn]
• Verfasst von: Brenner, Thorsten [VerfasserIn]
• Verfasst von: Fiedler-Kalenka, Mascha [VerfasserIn]
• Verfasst von: Hölle, Tobias [VerfasserIn]
• Verfasst von: Forst, Maik von der [VerfasserIn]
• Verfasst von: Wolf, Robert Christian [VerfasserIn]
• Verfasst von: Kopitz, Jürgen [VerfasserIn]
• Verfasst von: Gebert, Johannes [VerfasserIn]
• Verfasst von: Weigand, Markus A. [VerfasserIn]
• Titel: Extracellular vesicles as possible plasma markers and mediators in patients with sepsis-associated delirium
• Titelzusatz: a pilot study
• Verf.angabe: Konstanze Plaschke, Thorsten Brenner, Mascha O. Fiedler, Tobias Hölle, Maik von der Forst, Robert Christian Wolf, Jürgen Kopitz, Johannes Gebert and Markus A. Weigand
• E-Jahr: 2023
• Jahr: 30 October 2023
• Umfang: 18 S.
• Illustrationen: Illustrationen
• Fussnoten: Gesehen am 06.05.2024
• Titel Quelle: Enthalten in: International journal of molecular sciences
• Ort Quelle: Basel : Molecular Diversity Preservation International, 2000
• Jahr Quelle: 2023
• Band/Heft Quelle: 24(2023), 21, Artikel-ID 15781, Seite 1-18
• ISSN Quelle: 1422-0067
• ISSN Quelle: 1661-6596
• DOI: doi:10.3390/ijms242115781
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: intensive care unit
• Sach-SW: plasma extracellular vesicles (EVs)
• Sach-SW: proteomics
• Sach-SW: sepsis-associated delirium (SAD)
• K10plus-PPN: 1887862366

Abstract

Patients with sepsis-associated delirium (SAD) show severe neurological impairment, often require an intensive care unit (ICU) stay and have a high risk of mortality. Hence, useful biomarkers for early detection of SAD are urgently needed. Extracellular vesicles (EVs) and their cargo are known to maintain normal physiology but also have been linked to numerous disease states. Here, we sought to identify differentially expressed proteins in plasma EVs from SAD patients as potential biomarkers for SAD. Plasma EVs from 11 SAD patients and 11 age-matched septic patients without delirium (non-SAD) were isolated by differential centrifugation, characterized by nanoparticle tracking analysis, transmission electron microscopy and Western blot analysis. Differential EV protein expression was determined by mass spectrometry and the resulting proteomes were characterized by Gene Ontology term and between-group statistics. As preliminary results because of the small group size, five distinct proteins showed significantly different expression pattern between SAD and non-SAD patients (p ≤ 0.05). In SAD patients, upregulated proteins included paraoxonase-1 (PON1), thrombospondin 1 (THBS1), and full fibrinogen gamma chain (FGG), whereas downregulated proteins comprised immunoglobulin (IgHV3) and complement subcomponent (C1QC). Thus, plasma EVs of SAD patients show significant changes in the expression of distinct proteins involved in immune system regulation and blood coagulation as well as in lipid metabolism in this pilot study. They might be a potential indicator for to the pathogenesis of SAD and thus warrant further examination as potential biomarkers, but further research is needed to expand on these findings in longitudinal study designs with larger samples and comprehensive polymodal data collection.