Severity of staphylococcus aureus infection of the skin is associated with inducibility of human β-defensin 3 but not human β-defensin 2

• Verfasst von: Zanger, Philipp [VerfasserIn]
• Verfasst von: Holzer, Johannes [VerfasserIn]
• Verfasst von: Schleucher, Regina [VerfasserIn]
• Verfasst von: Scherbaum, Helmut [VerfasserIn]
• Verfasst von: Schittek, Birgit [VerfasserIn]
• Verfasst von: Gabrysch, Sabine [VerfasserIn]
• Titel: Severity of staphylococcus aureus infection of the skin is associated with inducibility of human β-defensin 3 but not human β-defensin 2
• Verf.angabe: Philipp Zanger, Johannes Holzer, Regina Schleucher, Helmut Scherbaum, Birgit Schittek, and Sabine Gabrysch
• E-Jahr: 2010
• Jahr: July 2010
• Umfang: 6 S.
• Illustrationen: Diagramme
• Fussnoten: Gesehen am 08.05.2024
• Titel Quelle: Enthalten in: Infection and immunity
• Ort Quelle: Washington, DC : Soc., 1970
• Jahr Quelle: 2010
• Band/Heft Quelle: 78(2010), 7 vom: Juli, Seite 3112-3117
• ISSN Quelle: 1098-5522
• DOI: doi:10.1128/iai.00078-10
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1888104007

Abstract

Gram-positive bacteria are the predominant cause of skin infections. Antimicrobial peptides (AMPs) are believed to be of major importance in skin's innate defense against these pathogens. This study aimed at providing clinical evidence for the contribution of AMP inducibility to determining the severity of Gram-positive skin infection. Using real-time PCR, we determined the induction of human β-defensin 2 (HBD-2), HBD-3, and RNase 7 by comparing healthy and lesional mRNA levels in 32 patients with Gram-positive skin infection. We then examined whether AMP induction differed by disease severity, as measured by number of recurrences and need for surgical drainage in patients with Staphylococcus aureus-positive lesions. We found that HBD-2 and -3, but not RNase 7, mRNA expression was highly induced by Gram-positive bacterial infection in otherwise healthy skin. Less induction of HBD-3, but not HBD-2, was associated with more-severe S. aureus skin infection: HBD-3 mRNA levels were 11.4 times lower in patients with more than 6 recurrences (P = 0.01) and 8.8 times lower in patients reporting surgical drainage (P = 0.01) than in the respective baseline groups. This suggests that inducibility of HBD-3 influences the severity of Gram-positive skin infection in vivo. The physiological function of HBD-2 induction in this context remains unclear.