| |  Online-Ressource |
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| Verfasst von: | Matuschik, Laura [VerfasserIn]  |
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| | Riabov, Vladimir [VerfasserIn] |
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| | Schmuttermaier, Christina [VerfasserIn] |
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| | Sevastyanova, Tatyana [VerfasserIn] |
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| | Weiß, Christel [VerfasserIn] |
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| | Klüter, Harald [VerfasserIn] |
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| | Kzhyshkowska, Julia [VerfasserIn] |
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| Titel: | Hyperglycemia induces inflammatory response of human macrophages to CD163-mediated scavenging of hemoglobin-haptoglobin complexes |
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| Verf.angabe: | Laura Matuschik, Vladimir Riabov, Christina Schmuttermaier, Tatyana Sevastyanova, Christel Weiss, Harald Klüter and Julia Kzhyshkowska |
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| E-Jahr: | 2022 |
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| Jahr: | 26 January 2022 |
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| Umfang: | 19 S. |
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| Illustrationen: | Illustrationen |
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| Fussnoten: | Gesehen am 14.05.2024 |
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| Weitere Titel: | Titel des übergeordneten Special issue: Crosstalk between epigenetic and metabolic mechanisms in chronic inflammation |
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| Titel Quelle: | Enthalten in: International journal of molecular sciences |
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| Ort Quelle: | Basel : Molecular Diversity Preservation International, 2000 |
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| Jahr Quelle: | 2022 |
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| Band/Heft Quelle: | 23(2022), 3, Artikel-ID 1385, Seite 1-19 |
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| ISSN Quelle: | 1422-0067 |
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| | 1661-6596 |
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| Abstract: | Hyperglycemia, a hallmark of diabetes, can induce inflammatory programming of macrophages. The macrophage scavenger receptor CD163 internalizes and degrades hemoglobin-haptoglobin (Hb-Hp) complexes built due to intravascular hemolysis. Clinical studies have demonstrated a correlation between impaired scavenging of Hb-Hp complexes via CD163 and diabetic vascular complications. Our aim was to identify whether hyperglycemia is able to amplify inflammation via Hb-Hp complex interactions with the immune system. M(IFNγ), M(IL-4), and control M0 macrophages were differentiated out of primary human monocytes in normo- (5 mM) and hyperglycemic (25 mM) conditions. CD163 gene expression was decreased 5.53 times in M(IFNγ) with a further decrease of 1.99 times in hyperglycemia. Hyperglycemia suppressed CD163 surface expression in M(IFNγ) (1.43 times). Flow cytometry demonstrated no impairment of Hb-Hp uptake in hyperglycemia. However, hyperglycemia induced an inflammatory response of M(IFNγ) to Hb-Hp1-1 and Hb-Hp2-2 uptake with different dynamics. Hb-Hp1-1 uptake stimulated IL-6 release (3.03 times) after 6 h but suppressed secretion (5.78 times) after 24 h. Contrarily, Hb-Hp2-2 uptake did not affect IL-6 release after 6h but increased secretion after 24 h (3.06 times). Our data show that hyperglycemia induces an inflammatory response of innate immune cells to Hb-Hp1-1 and Hb-Hp2-2 uptake, converting the silent Hb-Hp complex clearance that prevents vascular damage into an inflammatory process, hereby increasing the susceptibility of diabetic patients to vascular complications. |
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| DOI: | doi:10.3390/ijms23031385 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext: https://doi.org/10.3390/ijms23031385 |
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| | kostenfrei: Volltext: https://www.mdpi.com/1422-0067/23/3/1385 |
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| | DOI: https://doi.org/10.3390/ijms23031385 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | CD163 |
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| | diabetes mellitus |
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| | hemoglobin-haptoglobin complexes |
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| | hyperglycemia |
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| | inflammation |
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| | macrophages |
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| | scavenger receptor |
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| K10plus-PPN: | 1888456299 |
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| Verknüpfungen: | → Zeitschrift |
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Hyperglycemia induces inflammatory response of human macrophages to CD163-mediated scavenging of hemoglobin-haptoglobin complexes / Matuschik, Laura [VerfasserIn]; 26 January 2022 (Online-Ressource)
