| |  Online-Ressource |
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| Verfasst von: | Blaskovic, Sanja [VerfasserIn]  |
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| | Anagnostopoulou, Pinelopi [VerfasserIn] |
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| | Borisova, Elena [VerfasserIn] |
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| | Schittny, Dominik [VerfasserIn] |
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| | Donati, Yves [VerfasserIn] |
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| | Haberthür, David [VerfasserIn] |
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| | Zhou-Suckow, Zhe [VerfasserIn] |
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| | Mall, Marcus A. [VerfasserIn] |
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| | Schlepütz, Christian M. [VerfasserIn] |
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| | Stampanoni, Marco [VerfasserIn] |
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| | Barazzone-Argiroffo, Constance [VerfasserIn] |
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| | Schittny, Johannes C. [VerfasserIn] |
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| Titel: | Airspace diameter map |
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| Titelzusatz: | a quantitative measurement of all pulmonary airspaces to characterize structural lung diseases |
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| Verf.angabe: | Sanja Blaskovic, Pinelopi Anagnostopoulou, Elena Borisova, Dominik Schittny, Yves Donati, David Haberthür, Zhe Zhou-Suckow, Marcus A. Mall, Christian M. Schlepütz, Marco Stampanoni, Constance Barazzone-Argiroffo and Johannes C. Schittny |
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| E-Jahr: | 2023 |
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| Jahr: | 28 September 2023 |
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| Umfang: | 22 S. |
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| Illustrationen: | Illustrationen |
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| Fussnoten: | Gesehen am 24.05.2024 |
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| Titel Quelle: | Enthalten in: Cells |
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| Ort Quelle: | Basel : MDPI, 2012 |
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| Jahr Quelle: | 2023 |
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| Band/Heft Quelle: | 12(2023), 19, Artikel-ID 2375, Seite 1-22 |
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| ISSN Quelle: | 2073-4409 |
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| Abstract: | (1) Background: Stereological estimations significantly contributed to our understanding of lung anatomy and physiology. Taking stereology fully 3-dimensional facilitates the estimation of novel parameters. (2) Methods: We developed a protocol for the analysis of all airspaces of an entire lung. It includes (i) high-resolution synchrotron radiation-based X-ray tomographic microscopy, (ii) image segmentation using the free machine-learning tool Ilastik and ImageJ, and (iii) calculation of the airspace diameter distribution using a diameter map function. To evaluate the new pipeline, lungs from adult mice with cystic fibrosis (CF)-like lung disease (βENaC-transgenic mice) or mice with elastase-induced emphysema were compared to healthy controls. (3) Results: We were able to show the distribution of airspace diameters throughout the entire lung, as well as separately for the conducting airways and the gas exchange area. In the pathobiological context, we observed an irregular widening of parenchymal airspaces in mice with CF-like lung disease and elastase-induced emphysema. Comparable results were obtained when analyzing lungs imaged with μCT, sugges-ting that our pipeline is applicable to different kinds of imaging modalities. (4) Conclusions: We conclude that the airspace diameter map is well suited for a detailed analysis of unevenly distri-buted structural alterations in chronic muco-obstructive lung diseases such as cystic fibrosis and COPD. |
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| DOI: | doi:10.3390/cells12192375 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.3390/cells12192375 |
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| | Volltext: https://www.mdpi.com/2073-4409/12/19/2375 |
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| | DOI: https://doi.org/10.3390/cells12192375 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | artificial intelligence |
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| | cystic fibrosis |
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| | image analysis |
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| | lung disease |
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| | micro-computed X-ray tomography/micro-CT |
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| | pulmonary emphysema |
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| | stereology |
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| | X-ray tomographic microscopy |
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| K10plus-PPN: | 1889736562 |
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| Verknüpfungen: | → Zeitschrift |
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Airspace diameter map / Blaskovic, Sanja [VerfasserIn]; 28 September 2023 (Online-Ressource)
