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| Verfasst von: | Koch, Jana [VerfasserIn]  |
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| | Xin, Qilin [VerfasserIn] |
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| | Obr, Martin [VerfasserIn] |
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| | Schäfer, Alicia [VerfasserIn] |
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| | Rolfs, Nina [VerfasserIn] |
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| | Anagho, Holda A. [VerfasserIn] |
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| | Kudulyte, Aiste [VerfasserIn] |
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| | Woltereck, Lea [VerfasserIn] |
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| | Kummer, Susann [VerfasserIn] |
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| | Campos, Joaquin [VerfasserIn] |
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| | Uckeley, Zina Maria [VerfasserIn] |
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| | Bell-Sakyi, Lesley [VerfasserIn] |
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| | Kräusslich, Hans-Georg [VerfasserIn] |
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| | Schur, Florian Km [VerfasserIn] |
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| | Acuna, Claudio [VerfasserIn] |
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| | Lozach, Pierre-Yves [VerfasserIn] |
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| Titel: | The phenuivirus Toscana virus makes an atypical use of vacuolar acidity to enter host cells |
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| Verf.angabe: | Jana Koch, Qilin Xin, Martin Obr, Alicia Schäfer, Nina Rolfs, Holda A. Anagho, Aiste Kudulyte, Lea Woltereck, Susann Kummer, Joaquin Campos, Zina M. Uckeley, Lesley Bell-Sakyi, Hans-Georg Kräusslich, Florian Km Schur, Claudio Acuna, Pierre-Yves Lozach |
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| E-Jahr: | 2023 |
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| Jahr: | 14 Aug 2023 |
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| Umfang: | 32 S. |
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| Illustrationen: | Illustrationen |
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| Fussnoten: | Gesehen am 11.06.2024 |
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| Titel Quelle: | Enthalten in: Public Library of SciencePLoS pathogens |
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| Ort Quelle: | Lawrence, Kan. : PLoS, 2005 |
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| Jahr Quelle: | 2023 |
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| Band/Heft Quelle: | 19(2023), 8, Artikel-ID e1011562, Seite 1-32 |
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| ISSN Quelle: | 1553-7374 |
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| Abstract: | Toscana virus is a major cause of arboviral disease in humans in the Mediterranean basin during summer. However, early virus-host cell interactions and entry mechanisms remain poorly characterized. Investigating iPSC-derived human neurons and cell lines, we found that virus binding to the cell surface was specific, and 50% of bound virions were endocytosed within 10 min. Virions entered Rab5a+ early endosomes and, subsequently, Rab7a+ and LAMP-1+ late endosomal compartments. Penetration required intact late endosomes and occurred within 30 min following internalization. Virus entry relied on vacuolar acidification, with an optimal pH for viral membrane fusion at pH 5.5. The pH threshold increased to 5.8 with longer pre-exposure of virions to the slightly acidic pH in early endosomes. Strikingly, the particles remained infectious after entering late endosomes with a pH below the fusion threshold. Overall, our study establishes Toscana virus as a late-penetrating virus and reveals an atypical use of vacuolar acidity by this virus to enter host cells. |
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| DOI: | doi:10.1371/journal.ppat.1011562 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext: https://doi.org/10.1371/journal.ppat.1011562 |
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| | kostenfrei: Volltext: https://journals.plos.org/plospathogens/article?id= 10.1371/journal.ppat.1011562 |
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| | DOI: https://doi.org/10.1371/journal.ppat.1011562 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Endocytosis |
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| | Endosomes |
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| | Humans |
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| | Hydrogen-ion concentration |
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| | Sandfly fever Naples virus |
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| | Vacuoles |
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| | Virus internalization |
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| K10plus-PPN: | 1891060201 |
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| Verknüpfungen: | → Zeitschrift |
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¬The¬ phenuivirus Toscana virus makes an atypical use of vacuolar acidity to enter host cells / Koch, Jana [VerfasserIn]; 14 Aug 2023 (Online-Ressource)
