Modification of cell surfaces by enzymatic introduction of special sialic acid analogues

• Verfasst von: Kosa, Renate E. [VerfasserIn]
• Verfasst von: Brossmer, Reinhard [VerfasserIn]
• Verfasst von: Groß, Hans Jürgen [VerfasserIn]
• Titel: Modification of cell surfaces by enzymatic introduction of special sialic acid analogues
• Verf.angabe: Renate E. Kosa, Reinhard Brossmer, Hans Jürgen Groß
• E-Jahr: 1993
• Jahr: 15 February 1993
• Umfang: 7 S.
• Fussnoten: Elektronische Reproduktion der Druck-Ausgabe 25. Mai 2002 ; Gesehen am 20.06.2024
• Titel Quelle: Enthalten in: Biochemical and biophysical research communications
• Ort Quelle: [Amsterdam] : Elsevier B.V., 1959
• Jahr Quelle: 1993
• Band/Heft Quelle: 190(1993), 3, Seite 914-920
• ISSN Quelle: 1090-2104
• DOI: doi:10.1006/bbrc.1993.1136
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1891948350

Abstract

Taking advantage of the defined acceptor specifity of rat liver α2,6-sialyltransferase, N-linked Ga1β1,4GlcNAc-sequences of glycoconjugates at the plasma membrane of viable human erythrorytes and lymphoblastic IN-9 cells were modified to terminate in three NeuAc analogues endowed with special bemchemical properties: sialidase-resistant 9-amino-NeuAc, O-acetylesterase-resistant 9-acetamido-NeuAc and photolabile 9-azido-NeuAc. Incorporation of the latter analogues and of parent NeuAc proceeded to similar values, whereas 9-amino-NeuAc yielded slightly reduced values.