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| Verfasst von: | Settele, Simon [VerfasserIn]  |
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| | Schrage, C. Alexander [VerfasserIn] |
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| | Jung, Sebastian [VerfasserIn] |
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| | Michel, Elena [VerfasserIn] |
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| | Li, Han [VerfasserIn] |
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| | Flavel, Benjamin S. [VerfasserIn] |
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| | Hashmi, A. Stephen K. [VerfasserIn] |
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| | Kruss, Sebastian [VerfasserIn] |
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| | Zaumseil, Jana [VerfasserIn] |
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| Titel: | Ratiometric fluorescent sensing of pyrophosphate with sp³-functionalized single-walled carbon nanotubes |
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| Verf.angabe: | Simon Settele, C. Alexander Schrage, Sebastian Jung, Elena Michel, Han Li, Benjamin S. Flavel, A. Stephen K. Hashmi, Sebastian Kruss & Jana Zaumseil |
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| E-Jahr: | 2024 |
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| Jahr: | 24 January 2024 |
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| Umfang: | 13 S. |
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| Illustrationen: | Illustrationen |
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| Fussnoten: | Gesehen am 21.06.2024 ; Im Titel ist die Zahl 3 hochgestellt |
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| Titel Quelle: | Enthalten in: Nature Communications |
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| Ort Quelle: | [London] : Springer Nature, 2010 |
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| Jahr Quelle: | 2024 |
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| Band/Heft Quelle: | 15(2024), Artikel-ID 706, Seite 1-13 |
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| ISSN Quelle: | 2041-1723 |
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| Abstract: | Inorganic pyrophosphate is a key molecule in many biological processes from DNA synthesis to cell metabolism. Here we introduce sp3-functionalized (6,5) single-walled carbon nanotubes (SWNTs) with red-shifted defect emission as near-infrared luminescent probes for the optical detection and quantification of inorganic pyrophosphate. The sensing scheme is based on the immobilization of Cu2+ ions on the SWNT surface promoted by coordination to covalently attached aryl alkyne groups and a triazole complex. The presence of Cu2+ ions on the SWNT surface causes fluorescence quenching via photoinduced electron transfer, which is reversed by copper-complexing analytes such as pyrophosphate. The differences in the fluorescence response of sp3-defect to pristine nanotube emission enables reproducible ratiometric measurements in a wide concentration window. Biocompatible, phospholipid-polyethylene glycol-coated SWNTs with such sp3 defects are employed for the detection of pyrophosphate in cell lysate and for monitoring the progress of DNA synthesis in a polymerase chain reaction. This robust ratiometric and near-infrared luminescent probe for pyrophosphate may serve as a starting point for the rational design of nanotube-based biosensors. |
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| DOI: | doi:10.1038/s41467-024-45052-1 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext: https://doi.org/10.1038/s41467-024-45052-1 |
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| | kostenfrei: Volltext: https://www.nature.com/articles/s41467-024-45052-1 |
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| | DOI: https://doi.org/10.1038/s41467-024-45052-1 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Bibliogr. Hinweis: | Forschungsdaten: Settele, Simon, 1995 - : Ratiometric fluorescent sensing of pyrophosphate with sp³-functionalized single-walled carbon nanotubes [data] |
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| Sach-SW: | Carbon nanotubes and fullerenes |
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| | Fluorescence spectroscopy |
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| | Fluorescent probes |
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| K10plus-PPN: | 1892000407 |
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| Verknüpfungen: | → Zeitschrift |
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Ratiometric fluorescent sensing of pyrophosphate with sp³-functionalized single-walled carbon nanotubes / Settele, Simon [VerfasserIn]; 24 January 2024 (Online-Ressource)
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