Interactions of the Trypanosoma brucei brucei zinc-finger-domain protein ZC3H28

• Verfasst von: Bishola Tshitenge, Tania [VerfasserIn]
• Verfasst von: Clayton, Christine [VerfasserIn]
• Titel: Interactions of the Trypanosoma brucei brucei zinc-finger-domain protein ZC3H28
• Verf.angabe: Tania Bishola Tshitenge and Christine Clayton
• E-Jahr: 2022
• Jahr: March 2022
• Umfang: 15 S.
• Illustrationen: Illustrationen
• Fussnoten: Zuerst online von Cambridge University Press publiziert: 2 November 2021 ; Gesehen am 24.06.2024
• Titel Quelle: Enthalten in: Parasitology
• Ort Quelle: Cambridge [u.a.] : Cambridge Univ. Press, 1908
• Jahr Quelle: 2022
• Band/Heft Quelle: 149(2022), 3 vom: März, Seite 356-370
• ISSN Quelle: 1469-8161
• DOI: doi:10.1017/S003118202100189X
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: mRNA decay
• Sach-SW: RNA-binding protein
• Sach-SW: translation
• Sach-SW: Trypanosoma brucei
• K10plus-PPN: 1892103311
• K10plus-PPN:
  Universitätsbibliothek
• Lokale URL UB: Zum Volltext

Abstract

, - In Trypanosoma brucei and related Kinetoplastids, regulation of gene expression occurs mostly post-transcriptionally, and RNA-binding proteins play a critical role in the regulation of mRNA and protein abundance. Trypanosoma brucei ZC3H28 is a 114 KDa cytoplasmic mRNA-binding protein with a single C(x)7C(x)5C(x)sH zinc finger at the C-terminus and numerous proline-, histidine- or glutamine-rich regions. ZC3H28 is essential for normal bloodstream-form trypanosome growth, and when tethered to a reporter mRNA, ZC3H28 increased reporter mRNA and protein levels. Purification of N-terminally tagged ZC3H28 followed by mass spectrometry showed enrichment of ribosomal proteins, various RNA-binding proteins including both poly(A) binding proteins, the translation initiation complex EIF4E4/EIF4G3, and the activator MKT1. Tagged ZC3H28 was preferentially associated with long RNAs that have low complexity sequences in their 3′-untranslated regions; their coding regions also have low ribosome densities. In agreement with the tethering results, after ZC3H28 depletion, the levels of a significant proportion of its bound mRNAs decreased. We suggest that ZC3H28 is implicated in the stabilization of long mRNAs that are poorly translated.