Enzymatic transfer of sialic acids modified at C-5 employing four different sialyltransferases

• Verfasst von: Groß, Hans Jürgen [VerfasserIn]
• Verfasst von: Brossmer, Reinhard [VerfasserIn]
• Titel: Enzymatic transfer of sialic acids modified at C-5 employing four different sialyltransferases
• Verf.angabe: H.J. Groß, R. Brossmer
• E-Jahr: 1995
• Jahr: December 1995
• Umfang: 8 S.
• Fussnoten: Gesehen am 27.06.2024
• Titel Quelle: Enthalten in: Glycoconjugate journal
• Ort Quelle: Dordrecht [u.a.] : Springer Science + Business Media B.V, 1984
• Jahr Quelle: 1995
• Band/Heft Quelle: 12(1995), 6, Seite 739-746
• ISSN Quelle: 1573-4986
• DOI: doi:10.1007/BF00731233
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: CMP-glycosides
• Sach-SW: resialylation
• Sach-SW: sialic acid analogues
• Sach-SW: sialyltransferases
• K10plus-PPN: 1892305844

Abstract

We present kinetic studies on the enzymatic transfer of several synthetic sialic acid analogues, modified at C-5, to distinct glycoprotein glycans by sialytransferases differing in acceptor- and linkage-specificity. Biochemical properties of sialic acids were modified by introducing formyl-, trifluoroacetyl-, benzyloxycarbonyl-, and aminoacetyl-groups to the amino group at C-5 of neuraminic acid. The latter substitution renders the corresponding α-glyocoside resistant towards sialidases. The respective CMP-sialic acid analogues were prepared by CMP-sialic acid synthase with a yield of 13-55%.