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Verfasst von:Fischer, Edgar [VerfasserIn]   i
 Brossmer, Reinhard [VerfasserIn]   i
Titel:Sialic acid-binding lectins
Titelzusatz:submolecular specificity and interaction with sialoglycoproteins and tumour cells
Verf.angabe:Edgar Fischer, Reinhard Brossmer
E-Jahr:1995
Jahr:October 1995
Umfang:7 S.
Fussnoten:Gesehen am 27.06.2024
Titel Quelle:Enthalten in: Glycoconjugate journal
Ort Quelle:Dordrecht [u.a.] : Springer Science + Business Media B.V, 1984
Jahr Quelle:1995
Band/Heft Quelle:12(1995), 5, Seite 707-713
ISSN Quelle:1573-4986
Abstract:We examined the specificity of limulin,Limax flavus agglutinin (LFA) andSambucus nigra agglutinin I (SNA I) at the submolecular level of sialic acid, and characterized their interactions with a panel of structurally distinct sialoglycoproteins. In haemagglutination inhibition assays NeuAc-α-glycosides were stronger inhibitors for limulin and LFA than nativeN-acetylneuraminic acid (NeuAc). TheN-acetyl of NeuAc was crucial for binding to both lectins. N-thioacetylated NeuAc lost affinity for LFA, but still bound to limulin. Thus, distinct intermolecular interactions are involved in binding of sialic acid to the lectins. The glyceryl side chain was required for interaction with LFA, but not with limulin. SNA I specifically bound NeuAcα2 → 6Galα1 → 4Glc, but not monomeric sialic acids. Limulin and LFA strongly interacted with O-chain glycoproteins, whereas SNA I preferred N-chain proteins that carry NeuAcα2 → 6 residues. The lectins were compared with those fromCepaea hortensis andTachypleus tridentatus (TTA) and to wheat-germ agglutinin, and were then used to probe tumour cell lines for cell surface sialylation. With the exception of TTA, all lectins interacted with the tumour cells. Limulin distinguished between the low (Eb) and highly (ESb) metastatic mouse lymphoma lines by selectively agglutinating sialidase-treated ESb cells.
DOI:doi:10.1007/BF00731268
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.1007/BF00731268
 DOI: https://doi.org/10.1007/BF00731268
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:haemagglutination inhibition
 lectin specificity
 sialic acids
 sialoglycoproteins
 tumour cells
K10plus-PPN:1892334429
Verknüpfungen:→ Zeitschrift

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