| |  Online-Ressource |
|---|
| Verfasst von: | Müller, Patrick [VerfasserIn]  |
|---|
| | La Cuesta Zuluaga, Juan Jacobo de [VerfasserIn] |
|---|
| | Kuhn, Michael [VerfasserIn] |
|---|
| | Baghai Arassi, Maral [VerfasserIn] |
|---|
| | Treis, Tim [VerfasserIn] |
|---|
| | Blasche, Sonja [VerfasserIn] |
|---|
| | Zimmermann, Michael [VerfasserIn] |
|---|
| | Bork, Peer [VerfasserIn] |
|---|
| | Patil, Kiran Raosaheb [VerfasserIn] |
|---|
| | Typas, Athanasios [VerfasserIn] |
|---|
| | Garcia-Santamarina, Sarela [VerfasserIn] |
|---|
| | Maier, Lisa [VerfasserIn] |
|---|
| Titel: | High-throughput anaerobic screening for identifying compounds acting against gut bacteria in monocultures or communities |
|---|
| Verf.angabe: | Patrick Müller, Jacobo de la Cuesta-Zuluaga, Michael Kuhn, Maral Baghai Arassi, Tim Treis, Sonja Blasche, Michael Zimmermann, Peer Bork, Kiran Raosaheb Patil, Athanasios Typas, Sarela Garcia-Santamarina & Lisa Maier |
|---|
| E-Jahr: | 2023 |
|---|
| Jahr: | 13 December 2023 |
|---|
| Umfang: | 38 S. |
|---|
| Fussnoten: | Gesehen am 27.06.2024 |
|---|
| Titel Quelle: | Enthalten in: Nature protocols |
|---|
| Ort Quelle: | Basingstoke : Nature Publishing Group, 2006 |
|---|
| Jahr Quelle: | 2024 |
|---|
| Band/Heft Quelle: | 19(2024), 3, Seite 668-705 |
|---|
| ISSN Quelle: | 1750-2799 |
|---|
| Abstract: | The human gut microbiome is a key contributor to health, and its perturbations are linked to many diseases. Small-molecule xenobiotics such as drugs, chemical pollutants and food additives can alter the microbiota composition and are now recognized as one of the main factors underlying microbiome diversity. Mapping the effects of such compounds on the gut microbiome is challenging because of the complexity of the community, anaerobic growth requirements of individual species and the large number of interactions that need to be quantitatively assessed. High-throughput screening setups offer a promising solution for probing the direct inhibitory effects of hundreds of xenobiotics on tens of anaerobic gut bacteria. When automated, such assays enable the cost-effective investigation of a wide range of compound-microbe combinations. We have developed an experimental setup and protocol that enables testing of up to 5,000 compounds on a target gut species under strict anaerobic conditions within 5 d. In addition, with minor modifications to the protocol, drug effects can be tested on microbial communities either assembled from isolates or obtained from stool samples. Experience in working in an anaerobic chamber, especially in performing delicate work with thick chamber gloves, is required for implementing this protocol. We anticipate that this protocol will accelerate the study of interactions between small molecules and the gut microbiome and provide a deeper understanding of this microbial ecosystem, which is intimately intertwined with human health. |
|---|
| DOI: | doi:10.1038/s41596-023-00926-4 |
|---|
| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1038/s41596-023-00926-4 |
|---|
| | Volltext: https://www.nature.com/articles/s41596-023-00926-4 |
|---|
| | DOI: https://doi.org/10.1038/s41596-023-00926-4 |
|---|
| Datenträger: | Online-Ressource |
|---|
| Sprache: | eng |
|---|
| Sach-SW: | Antimicrobials |
|---|
| | Microbiome |
|---|
| K10plus-PPN: | 1892344955 |
|---|
| Verknüpfungen: | → Zeitschrift |
|---|
High-throughput anaerobic screening for identifying compounds acting against gut bacteria in monocultures or communities / Müller, Patrick [VerfasserIn]; 13 December 2023 (Online-Ressource)
