| |  Online-Ressource |
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| Verfasst von: | Kawaguchi, Yohei [VerfasserIn]  |
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| | Hagiwara, Daisuke [VerfasserIn] |
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| | Tsumura, Tetsuro [VerfasserIn] |
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| | Miyata, Takashi [VerfasserIn] |
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| | Kobayashi, Tomoko [VerfasserIn] |
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| | Sugiyama, Mariko [VerfasserIn] |
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| | Onoue, Takeshi [VerfasserIn] |
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| | Yasuda, Yoshinori [VerfasserIn] |
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| | Iwama, Shintaro [VerfasserIn] |
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| | Suga, Hidetaka [VerfasserIn] |
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| | Banno, Ryoichi [VerfasserIn] |
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| | Grinevich, Valéry [VerfasserIn] |
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| | Arima, Hiroshi [VerfasserIn] |
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| Titel: | Knockdown of endoplasmic reticulum chaperone BiP leads to the death of parvocellular AVP/CRH neurons in mice |
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| Verf.angabe: | Yohei Kawaguchi, Daisuke Hagiwara, Tetsuro Tsumura, Takashi Miyata, Tomoko Kobayashi, Mariko Sugiyama, Takeshi Onoue, Yoshinori Yasuda, Shintaro Iwama, Hidetaka Suga, Ryoichi Banno, Valery Grinevich, Hiroshi Arima |
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| E-Jahr: | 2023 |
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| Jahr: | January 2023 |
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| Umfang: | 10 S. |
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| Fussnoten: | Erstmals veröffentlicht: 01. Dezember 2022 ; Gesehen am 01.07.2024 |
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| Titel Quelle: | Enthalten in: Journal of neuroendocrinology |
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| Ort Quelle: | Oxford [u.a.] : Wiley-Blackwell, 1989 |
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| Jahr Quelle: | 2023 |
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| Band/Heft Quelle: | 35(2023), 1 vom: Jan., Artikel-ID e13223, Seite 1-10 |
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| ISSN Quelle: | 1365-2826 |
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| Abstract: | Arginine vasopressin (AVP) is expressed in both magnocellular (magnAVP) and parvocellular AVP (parvAVP) neurons of the paraventricular nucleus, and AVP colocalizes with corticotropin-releasing hormone (CRH) only in the parvocellular neurons. The immunoglobulin heavy chain binding protein (BiP) is a major endoplasmic reticulum (ER) chaperone which regulates the unfolded protein response under ER stress. We previously demonstrated that knockdown of BiP in magnAVP neurons exacerbated ER stress, which resulted in the autophagy-associated cell death of magnAVP neurons. Using the same approach, in the present study we examined the role of BiP in mouse parvAVP/CRH neurons. Our data demonstrate that BiP is expressed in mouse parvAVP/CRH neurons under nonstress conditions and is upregulated in proportion to the increase in CRH expression after adrenalectomy. For BiP knockdown in parvAVP/CRH neurons, we utilized a viral approach in combination with shRNA interference. Knockdown of BiP expression induced ER stress in parvAVP/CRH neurons, as reflected by the expression of C/EBP homologous protein. Furthermore, BiP knockdown led to the loss of parvAVP/CRH neurons after 4 weeks. In summary, our results demonstrate that BiP plays a pivotal role in parvAVP/CRH neurons, which function as neuroendocrine cells producing a large number of secretory proteins. |
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| DOI: | doi:10.1111/jne.13223 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1111/jne.13223 |
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| | Volltext: http://onlinelibrary.wiley.com/doi/abs/10.1111/jne.13223 |
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| | DOI: https://doi.org/10.1111/jne.13223 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | arginine vasopressin |
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| | corticotropin-releasing hormone |
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| | endoplasmic reticulum stress |
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| | immunoglobulin heavy chain binding protein |
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| | parvocellular neuron |
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| K10plus-PPN: | 1892787725 |
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| Verknüpfungen: | → Zeitschrift |
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Knockdown of endoplasmic reticulum chaperone BiP leads to the death of parvocellular AVP/CRH neurons in mice / Kawaguchi, Yohei [VerfasserIn]; January 2023 (Online-Ressource)
