Electroconvulsive stimulation in rats induces alterations in the hippocampal miRNome

• Verfasst von: Ryan, Karen M. [VerfasserIn]
• Verfasst von: Smyth, Paul [VerfasserIn]
• Verfasst von: Blackshields, Gordon [VerfasserIn]
• Verfasst von: Kranaster, Laura [VerfasserIn]
• Verfasst von: Sartorius, Alexander [VerfasserIn]
• Verfasst von: Sheils, Orla [VerfasserIn]
• Verfasst von: McLoughlin, Declan M. [VerfasserIn]
• Titel: Electroconvulsive stimulation in rats induces alterations in the hippocampal miRNome
• Titelzusatz: translational implications for depression
• Verf.angabe: Karen M. Ryan, Paul Smyth, Gordon Blackshields, Laura Kranaster, Alexander Sartorius, Orla Sheils, Declan M. McLoughlin
• E-Jahr: 2023
• Jahr: March 2023
• Umfang: 14 S.
• Fussnoten: Online veröffentlicht: 22. November 2022 ; Gesehen am 23.07.2024
• Titel Quelle: Enthalten in: Molecular neurobiology
• Ort Quelle: Totowa, NJ : Humana Press, 1987
• Jahr Quelle: 2023
• Band/Heft Quelle: 60(2023), 3 vom: März, Seite 1150-1163
• ISSN Quelle: 1559-1182
• DOI: doi:10.1007/s12035-022-03131-8
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Depression
• Sach-SW: Electroconvulsive stimulation
• Sach-SW: Electroconvulsive therapy
• Sach-SW: microRNA
• Sach-SW: Next-generation sequencing
• K10plus-PPN: 1896032168

Abstract

MicroRNAs (miRNAs) may contribute to the development of depression and its treatment. Here, we used the hypothesis-neutral approach of next-generation sequencing (NGS) to gain comprehensive understanding of the effects of a course of electroconvulsive stimulation (ECS), the animal model equivalent of electroconvulsive therapy (ECT), on rat hippocampal miRNAs. Significant differential expression (p < 0.001) of six hippocampal miRNAs was noted following NGS, after correcting for multiple comparisons. Three of these miRNAs were upregulated (miR-132, miR-212, miR-331) and three downregulated (miR-204, miR-483, miR-301a). qRT-PCR confirmed significant changes in four of the six miRNAs (miR-132, miR-212, miR-204, miR-483). miR-483 was also significantly reduced in frontal cortex, though no other significant alterations were noted in frontal cortex, cerebellum, or whole blood. Assessing the translatability of the results, miR-132 and miR-483 were significantly reduced in whole blood samples from medicated patients with depression (n = 50) compared to healthy controls (n = 45), though ECT had no impact on miRNA levels. Notably, pre-ECT miR-204 levels moderately positively correlated with depression severity at baseline and moderately negatively correlated with mood score reduction post-ECT. miRNAs were also examined in cerebrospinal fluid and serum from a separate cohort of patients (n = 8) treated with ECT; no significant changes were noted post-treatment. However, there was a large positive correlation between changes in miR-212 and mood score post-ECT in serum. Though replication studies using larger sample sizes are required, alterations in miRNA expression may be informative about the mechanism of action of ECS/ECT and in turn might give insight into the neurobiology of depression.