Navigation überspringen
Universitätsbibliothek Heidelberg
Status: Bibliographieeintrag

Verfügbarkeit
Standort: ---
Exemplare: ---
heiBIB
 Online-Ressource
Verfasst von:Gamez, Carolina [VerfasserIn]   i
 Schneider-Wald, Barbara Katharina [VerfasserIn]   i
 Bieback, Karen [VerfasserIn]   i
 Schütte, Andy [VerfasserIn]   i
 Büttner, Sylvia [VerfasserIn]   i
 Hafner, Mathias [VerfasserIn]   i
 Gretz, Norbert [VerfasserIn]   i
 Schwarz, Markus [VerfasserIn]   i
Titel:Compression bioreactor-based mechanical loading induces mobilization of Human bone Marrow-Derived Mesenchymal stromal cells into collagen scaffolds in vitro
Verf.angabe:Carolina Gamez, Barbara Schneider-Wald, Karen Bieback, Andy Schuette, Sylvia Büttner, Mathias Hafner, Norbert Gretz and Markus L. Schwarz
E-Jahr:2020
Jahr:4 November 2020
Umfang:17 S.
Illustrationen:Illustrationen
Fussnoten:Gesehen am 29.07.2024
Titel Quelle:Enthalten in: International journal of molecular sciences
Ort Quelle:Basel : Molecular Diversity Preservation International, 2000
Jahr Quelle:2020
Band/Heft Quelle:21(2020), 21 vom: Nov., Artikel-ID 8249, Seite 1-17
ISSN Quelle:1422-0067
 1661-6596
Abstract:Articular cartilage (AC) is an avascular tissue composed of scattered chondrocytes embedded in a dense extracellular matrix, in which nourishment takes place via the synovial fluid at the surface. AC has a limited intrinsic healing capacity, and thus mainly surgical techniques have been used to relieve pain and improve function. Approaches to promote regeneration remain challenging. The microfracture (MF) approach targets the bone marrow (BM) as a source of factors and progenitor cells to heal chondral defects in situ by opening small holes in the subchondral bone. However, the original function of AC is not obtained yet. We hypothesize that mechanical stimulation can mobilize mesenchymal stromal cells (MSCs) from BM reservoirs upon MF of the subchondral bone. Thus, the aim of this study was to compare the counts of mobilized human BM-MSCs (hBM-MSCs) in alginate-laminin (alginate-Ln) or collagen-I (col-I) scaffolds upon intermittent mechanical loading. The mechanical set up within an established bioreactor consisted of 10% strain, 0.3 Hz, breaks of 10 s every 180 cycles for 24 h. Contrary to previous findings using porcine MSCs, no significant cell count was found for hBM-MSCs into alginate-Ln scaffolds upon mechanical stimulation (8 ± 5 viable cells/mm3 for loaded and 4 ± 2 viable cells/mm3 for unloaded alginate-Ln scaffolds). However, intermittent mechanical stimulation induced the mobilization of hBM-MSCs into col-I scaffolds 10-fold compared to the unloaded col-I controls (245 ± 42 viable cells/mm3 vs. 22 ± 6 viable cells/mm3, respectively; p-value < 0.0001). Cells that mobilized into the scaffolds by mechanical loading did not show morphological changes. This study confirmed that hBM-MSCs can be mobilized in vitro from a reservoir toward col-I but not alginate-Ln scaffolds upon intermittent mechanical loading, against gravity.
DOI:doi:10.3390/ijms21218249
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

kostenfrei: Volltext: https://doi.org/10.3390/ijms21218249
 kostenfrei: Volltext: https://www.mdpi.com/1422-0067/21/21/8249
 DOI: https://doi.org/10.3390/ijms21218249
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:alginate-laminin scaffolds
 articular cartilage regeneration
 cell mobilization
 collagen scaffolds
 compression bioreactor
 intermittent mechanical stimulation
 mechanical loading
 mesenchymal stromal cells
 MSCs
K10plus-PPN:1896788858
Verknüpfungen:→ Zeitschrift

Permanenter Link auf diesen Titel (bookmarkfähig):  https://katalog.ub.uni-heidelberg.de/titel/69238120   QR-Code
zum Seitenanfang