| |  Online-Ressource |
|---|
| Verfasst von: | Schnitter, Florian [VerfasserIn]  |
|---|
| | Stangl, Franziska [VerfasserIn] |
|---|
| | Noeske, Elisabeth [VerfasserIn] |
|---|
| | Bille, Maya [VerfasserIn] |
|---|
| | Stadtmüller, Anja [VerfasserIn] |
|---|
| | Vogt, Niklas [VerfasserIn] |
|---|
| | Sicklinger, Florian [VerfasserIn] |
|---|
| | Leuschner, Florian [VerfasserIn] |
|---|
| | Frey, Anna [VerfasserIn] |
|---|
| | Schreiber, Laura [VerfasserIn] |
|---|
| | Frantz, Stefan [VerfasserIn] |
|---|
| | Beyersdorf, Niklas [VerfasserIn] |
|---|
| | Ramos, Gustavo [VerfasserIn] |
|---|
| | Gladow, Nadine [VerfasserIn] |
|---|
| | Hofmann, Ulrich [VerfasserIn] |
|---|
| Titel: | Characterizing the immune response to myocardial infarction in pigs |
|---|
| Verf.angabe: | Florian Schnitter, Franziska Stangl, Elisabeth Noeske, Maya Bille, Anja Stadtmüller, Niklas Vogt, Florian Sicklinger, Florian Leuschner, Anna Frey, Laura Schreiber, Stefan Frantz, Niklas Beyersdorf, Gustavo Ramos, Nadine Gladow, Ulrich Hofmann |
|---|
| E-Jahr: | 2024 |
|---|
| Jahr: | 15 March 2024 |
|---|
| Umfang: | 27 S. |
|---|
| Illustrationen: | Illustrationen |
|---|
| Fussnoten: | Gesehen am 30.07.2024 |
|---|
| Titel Quelle: | Enthalten in: Basic research in cardiology |
|---|
| Ort Quelle: | [Darmstadt u.a.] : Steinkopff, 1937 |
|---|
| Jahr Quelle: | 2024 |
|---|
| Band/Heft Quelle: | 119(2024), 3, Seite 453-479 |
|---|
| ISSN Quelle: | 1435-1803 |
|---|
| Abstract: | Though myocardial infarction (MI) in pigs is a well-established translational large animal model, it has not yet been widely used for immunotherapy studies, and a comprehensive description of the immune response to MI in this species is lacking. We induced MI in Landrace pigs by balloon occlusion of the left anterior descending artery over 90 min. Within 14 days, the necrotic myocardium was progressively replaced by scar tissue with involvement of myofibroblasts. We characterized the immune response in the heart ex vivo by (immuno)histology, flow cytometry, and RNA sequencing of myocardial tissue on days 3, 7, and 14 after MI. Besides a clear predominance of myeloid cells among heart-infiltrating leukocytes, we detected activated T cells and an increasing proportion of CD4+ Foxp3+ regulatory T cells (Treg), especially in the infarct core—findings that closely mirror what has been observed in mice and humans after MI. Transcriptome data indicated inflammatory activity that was persistent but markedly changing in character over time and linked to extracellular matrix biology. Analysis of lymphocytes in heart-draining lymph nodes revealed significantly higher proliferation rates of T helper cell subsets, including Treg on day 7 after MI, compared to sham controls. Elevated frequencies of myeloid progenitors in the spleen suggest that it might be a site of emergency myelopoiesis after MI in pigs, as previously shown in mice. We thus provide a first description of the immune response to MI in pigs, and our results can aid future research using the species for preclinical immunotherapy studies. |
|---|
| DOI: | doi:10.1007/s00395-024-01036-2 |
|---|
| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext: https://doi.org/10.1007/s00395-024-01036-2 |
|---|
| | DOI: https://doi.org/10.1007/s00395-024-01036-2 |
|---|
| Datenträger: | Online-Ressource |
|---|
| Sprache: | eng |
|---|
| Sach-SW: | Heart |
|---|
| | Immunophenotyping |
|---|
| | Leukocytes |
|---|
| | Myocardial infarction |
|---|
| | Pig |
|---|
| | Swine |
|---|
| K10plus-PPN: | 1896922554 |
|---|
| Verknüpfungen: | → Zeitschrift |
|---|
Characterizing the immune response to myocardial infarction in pigs / Schnitter, Florian [VerfasserIn]; 15 March 2024 (Online-Ressource)
