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| Verfasst von: | Bergs, Amelie [VerfasserIn]  |
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| | Liewald, Jana F. [VerfasserIn] |
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| | Rodriguez-Rozada, Silvia [VerfasserIn] |
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| | Liu, Qiang [VerfasserIn] |
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| | Wirt, Christin [VerfasserIn] |
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| | Bessel, Artur [VerfasserIn] |
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| | Zeitzschel, Nadja [VerfasserIn] |
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| | Durmaz, Hilal [VerfasserIn] |
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| | Nozownik, Adrianna [VerfasserIn] |
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| | Dill, Holger [VerfasserIn] |
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| | Jospin, Maëlle [VerfasserIn] |
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| | Vierock, Johannes [VerfasserIn] |
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| | Bargmann, Cornelia I. [VerfasserIn] |
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| | Hegemann, Peter [VerfasserIn] |
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| | Wiegert, Simon [VerfasserIn] |
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| | Gottschalk, Alexander [VerfasserIn] |
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| Titel: | All-optical closed-loop voltage clamp for precise control of muscles and neurons in live animals |
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| Verf.angabe: | Amelie C.F. Bergs, Jana F. Liewald, Silvia Rodriguez-Rozada, Qiang Liu, Christin Wirt, Artur Bessel, Nadja Zeitzschel, Hilal Durmaz, Adrianna Nozownik, Holger Dill, Maëlle Jospin, Johannes Vierock, Cornelia I. Bargmann, Peter Hegemann, J. Simon Wiegert & Alexander Gottschalk |
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| E-Jahr: | 2023 |
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| Jahr: | 06 April 2023 |
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| Umfang: | 17 S. |
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| Illustrationen: | Illustrationen |
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| Fussnoten: | Gesehen am 02.08.2024 |
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| Titel Quelle: | Enthalten in: Nature Communications |
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| Ort Quelle: | [London] : Springer Nature, 2010 |
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| Jahr Quelle: | 2023 |
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| Band/Heft Quelle: | 14(2023), Artikel-ID 1939, Seite 1-17 |
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| ISSN Quelle: | 2041-1723 |
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| Abstract: | Excitable cells can be stimulated or inhibited by optogenetics. Since optogenetic actuation regimes are often static, neurons and circuits can quickly adapt, allowing perturbation, but not true control. Hence, we established an optogenetic voltage-clamp (OVC). The voltage-indicator QuasAr2 provides information for fast, closed-loop optical feedback to the bidirectional optogenetic actuator BiPOLES. Voltage-dependent fluorescence is held within tight margins, thus clamping the cell to distinct potentials. We established the OVC in muscles and neurons of Caenorhabditis elegans, and transferred it to rat hippocampal neurons in slice culture. Fluorescence signals were calibrated to electrically measured potentials, and wavelengths to currents, enabling to determine optical I/V-relationships. The OVC reports on homeostatically altered cellular physiology in mutants and on Ca2+-channel properties, and can dynamically clamp spiking in C. elegans. Combining non-invasive imaging with control capabilities of electrophysiology, the OVC facilitates high-throughput, contact-less electrophysiology in individual cells and paves the way for true optogenetic control in behaving animals. |
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| DOI: | doi:10.1038/s41467-023-37622-6 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext: https://doi.org/10.1038/s41467-023-37622-6 |
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| | kostenfrei: Volltext: https://www.nature.com/articles/s41467-023-37622-6 |
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| | DOI: https://doi.org/10.1038/s41467-023-37622-6 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Assay systems |
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| | Ion channels in the nervous system |
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| | Optogenetics |
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| K10plus-PPN: | 1897498012 |
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| Verknüpfungen: | → Zeitschrift |
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All-optical closed-loop voltage clamp for precise control of muscles and neurons in live animals / Bergs, Amelie [VerfasserIn]; 06 April 2023 (Online-Ressource)
