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| Verfasst von: | Hofmann, Annika [VerfasserIn]  |
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| | Krajnc, Nik [VerfasserIn] |
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| | Dal-Bianco, Assunta [VerfasserIn] |
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| | Riedl, Christian J. [VerfasserIn] |
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| | Zrzavy, Tobias [VerfasserIn] |
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| | Lerma Martin, Celia [VerfasserIn] |
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| | Kasprian, Gregor [VerfasserIn] |
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| | Weber, Claudia E. [VerfasserIn] |
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| | Pezzini, Francesco [VerfasserIn] |
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| | Leutmezer, Fritz [VerfasserIn] |
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| | Rommer, Paulus [VerfasserIn] |
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| | Bsteh, Gabriel [VerfasserIn] |
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| | Platten, Michael [VerfasserIn] |
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| | Gass, Achim [VerfasserIn] |
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| | Berger, Thomas [VerfasserIn] |
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| | Eisele, Philipp [VerfasserIn] |
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| | Magliozzi, Roberta [VerfasserIn] |
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| | Schirmer, Lucas [VerfasserIn] |
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| | Hametner, Simon [VerfasserIn] |
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| Titel: | Myeloid cell iron uptake pathways and paramagnetic rim formation in multiple sclerosis |
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| Verf.angabe: | Annika Hofmann, Nik Krajnc, Assunta Dal-Bianco, Christian J. Riedl, Tobias Zrzavy, Celia Lerma-Martin, Gregor Kasprian, Claudia E. Weber, Francesco Pezzini, Fritz Leutmezer, Paulus Rommer, Gabriel Bsteh, Michael Platten, Achim Gass, Thomas Berger, Philipp Eisele, Roberta Magliozzi, Lucas Schirmer, Simon Hametner |
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| E-Jahr: | 2023 |
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| Jahr: | November 2023 |
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| Umfang: | 18 S. |
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| Fussnoten: | Online veröffentlicht: 16. September 2023 ; Gesehen am 06.08.2024 |
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| Titel Quelle: | Enthalten in: Acta neuropathologica |
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| Ort Quelle: | Berlin : Springer, 1961 |
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| Jahr Quelle: | 2023 |
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| Band/Heft Quelle: | 146(2023), 5, Seite 707-724 |
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| ISSN Quelle: | 1432-0533 |
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| Abstract: | In multiple sclerosis (MS), sustained inflammatory activity can be visualized by iron-sensitive magnetic resonance imaging (MRI) at the edges of chronic lesions. These paramagnetic rim lesions (PRLs) are associated with clinical worsening, although the cell type-specific and molecular pathways of iron uptake and metabolism are not well known. We studied two postmortem cohorts: an exploratory formalin-fixed paraffin-embedded (FFPE) tissue cohort of 18 controls and 24 MS cases and a confirmatory snap-frozen cohort of 6 controls and 14 MS cases. Besides myelin and non-heme iron imaging, the haptoglobin-hemoglobin scavenger receptor CD163, the iron-metabolizing markers HMOX1 and HAMP as well as immune-related markers P2RY12, CD68, C1QA and IL10 were visualized in myeloid cell (MC) subtypes at RNA and protein levels across different MS lesion areas. In addition, we studied PRLs in vivo in a cohort of 98 people with MS (pwMS) via iron-sensitive 3 T MRI and haptoglobin genotyping by PCR. CSF samples were available from 38 pwMS for soluble CD163 (sCD163) protein level measurements by ELISA. In postmortem tissues, we observed that iron uptake was linked to rim-associated C1QA-expressing MC subtypes, characterized by upregulation of CD163, HMOX1, HAMP and, conversely, downregulation of P2RY12. We found that pwMS with $$\ge |
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| DOI: | doi:10.1007/s00401-023-02627-4 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext: https://doi.org/10.1007/s00401-023-02627-4 |
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| | kostenfrei: Volltext: https://link.springer.com/article/10.1007/s00401-023-02627-4 |
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| | DOI: https://doi.org/10.1007/s00401-023-02627-4 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | CD163 |
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| | Haptoglobin |
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| | Iron metabolism |
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| | Magnetic resonance imaging |
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| | Multiple sclerosis |
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| | Postmortem |
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| | White matter |
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| K10plus-PPN: | 1897878044 |
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| Verknüpfungen: | → Zeitschrift |
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Myeloid cell iron uptake pathways and paramagnetic rim formation in multiple sclerosis / Hofmann, Annika [VerfasserIn]; November 2023 (Online-Ressource)
