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Verfasst von:Tengler, Luisa [VerfasserIn]   i
 Schütz, Julia [VerfasserIn]   i
 Tiedtke, Moritz [VerfasserIn]   i
 Jablonska, Jadwiga [VerfasserIn]   i
 Theodoraki, Marie-Nicole [VerfasserIn]   i
 Nitschke, Katja [VerfasserIn]   i
 Weiß, Christel [VerfasserIn]   i
 Seiz, Elena [VerfasserIn]   i
 Affolter, Annette [VerfasserIn]   i
 Jungbauer, Frederic [VerfasserIn]   i
 Lammert, Anne [VerfasserIn]   i
 Rotter, Nicole [VerfasserIn]   i
 Ludwig, Sonja [VerfasserIn]   i
Titel:Plasma-derived small extracellular vesicles unleash the angiogenic potential in head and neck cancer patients
Verf.angabe:Luisa Tengler, Julia Schütz, Moritz Tiedtke, Jadwiga Jablonska, Marie-Nicole Theodoraki, Katja Nitschke, Christel Weiß, Elena Seiz, Annette Affolter, Frederic Jungbauer, Anne Lammert, Nicole Rotter and Sonja Ludwig
E-Jahr:2023
Jahr:24 May 2023
Umfang:14 S.
Illustrationen:Illustrationen
Fussnoten:Gesehen am 12.08.2024
Titel Quelle:Enthalten in: Molecular medicine
Ort Quelle:[London] : BioMed Central, 1994
Jahr Quelle:2023
Band/Heft Quelle:29(2023), 1, Seite 1-14
ISSN Quelle:1528-3658
Abstract:In Head and neck cancer (HNC) angiogenesis is essential for tumor progression and metastasis. Small extracellular vesicles (sEVs) from HNC cell lines alter endothelial cell (EC) functions towards a pro-angiogenic phenotype. However, the role of plasma sEVs retrieved from HNC patients in this process is not clear so far. Plasma sEVs were isolated on size exclusion chromatography columns from 32 HNC patients (early-stage UICC I/II: 8, advanced-stage UICC III/IV: 24), 12 patients with no evident disease after therapy (NED) and 16 healthy donors (HD). Briefly, sEVs were characterized by transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA), BCA protein assays and Western blots. Levels of angiogenesis-associated proteins were determined using antibody arrays. The interaction of fluorescently-labeled sEVs with human umbilical vein ECs was visualized by confocal microscopy. The functional effect of sEVs on tubulogenesis, migration, proliferation and apoptosis of ECs was assessed. The internalization of sEVs by ECs was visualized using confocal microscopy. Based on antibody arrays, all plasma sEVs were enriched in anti-angiogenic proteins. HNC sEVs contained more pro-angiogenic MMP-9 and anti-angiogenic proteins (Serpin F1) than HD sEVs. Interestingly, a strong inhibition of EC function was observed for sEVs from early-stage HNC, NED and HD. In contrast, sEVs from advanced-stage HNC showed a significantly increased tubulogenesis, migration and proliferation and induced less apoptosis in ECs than sEVs from HD. In general, plasma sEVs carry a predominantly anti-angiogenic protein cargo and suppress the angiogenic properties of ECs, while sEVs from (advanced-stage) HNC patients induce angiogenesis compared to HD sEVs. Thus, tumor-derived sEVs within the plasma of HNC patients might shift the angiogenic switch towards angiogenesis.
DOI:doi:10.1186/s10020-023-00659-w
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

kostenfrei: Volltext: https://doi.org/10.1186/s10020-023-00659-w
 kostenfrei: Volltext: https://molmed.biomedcentral.com/articles/10.1186/s10020-023-00659-w
 DOI: https://doi.org/10.1186/s10020-023-00659-w
Datenträger:Online-Ressource
Sprache:eng
K10plus-PPN:1898407649
Verknüpfungen:→ Zeitschrift

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