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Verfasst von:Begni, Veronica [VerfasserIn]   i
 Sanson, Alice [VerfasserIn]   i
 Pfeiffer, Natascha [VerfasserIn]   i
 Brandwein, Christiane [VerfasserIn]   i
 Inta, Dragos [VerfasserIn]   i
 Talbot, Steven R. [VerfasserIn]   i
 Riva, Marco Andrea [VerfasserIn]   i
 Gass, Peter [VerfasserIn]   i
 Mallien, Anne Stephanie [VerfasserIn]   i
Titel:Social isolation in rats
Titelzusatz:effects on animal welfare and molecular markers for neuroplasticity
Verf.angabe:Veronica Begni, Alice Sanson, Natascha Pfeiffer, Christiane Brandwein, Dragos Inta, Steven R. Talbot, Marco Andrea Riva, Peter Gass, Anne Stephanie Mallien
E-Jahr:2020
Jahr:October 27, 2020
Umfang:25 S.
Fussnoten:Gesehen am 12.08.2024
Titel Quelle:Enthalten in: PLOS ONE
Ort Quelle:San Francisco, California, US : PLOS, 2006
Jahr Quelle:2020
Band/Heft Quelle:15(2020), 10 vom: Okt., Artikel-ID e0240439, Seite 1-25
ISSN Quelle:1932-6203
Abstract:Early life stress compromises brain development and can contribute to the development of mental illnesses. A common animal model used to study different facets of psychiatric disorders is social isolation from early life on. In rats, this isolation can induce long-lasting alterations in molecular expression and in behavior. Since social isolation models severe psychiatric symptoms, it is to be expected that it affects the overall wellbeing of the animals. As also promoted by the 3Rs principle, though, it is pivotal to decrease the burden of laboratory animals by limiting the number of subjects (reduce, replace) and by improving the animals’ wellbeing (refine). The aim of this study was therefore to test possible refinement strategies such as resocialization and mere adult social isolation. We examined whether the alternatives still triggered the necessary phenotype while minimizing the stress load on the animals. Interestingly, we did not find reduced wellbeing-associated burrowing performance in isolated rats. The hyperactive phenotype seen in socially isolated animals was observed for rats undergoing the adult-only isolation, but resocializing ameliorated the locomotor abnormality. Isolation strongly affected markers of neuroplasticity in the prefrontal cortex independent of timing: mRNA levels of Arc, Bdnf and the pool of Bdnf transcripts with the 3’ long UTR were reduced in all groups. Bdnf splice variant IV expression was reduced in lifelong-isolated animals. Some of these deficits normalized after resocialization; likewise, exon VI Bdnf mRNA levels were reduced only in animals persistently isolated. Conversely, social deprivation did not affect the expression of Gad67 and Pvb, two GABAergic markers, whereas changes occurred in the expression of dopamine d1 and d2 receptors. As adult isolation was sufficient to trigger the hyperactive phenotype and impaired neuroplasticity in the prefrontal cortex, it could be a candidate for a refinement strategy for certain research questions. To fully grade the severity of post-weaning social isolation and the alternatives, adult isolation and resocialization, a more profound and multimodal assessment approach is necessary.
DOI:doi:10.1371/journal.pone.0240439
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

kostenfrei: Volltext: https://doi.org/10.1371/journal.pone.0240439
 kostenfrei: Volltext: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0240439
 DOI: https://doi.org/10.1371/journal.pone.0240439
Datenträger:Online-Ressource
Sprache:eng
Bibliogr. Hinweis:Errata: Begni, Veronica: Correction: social isolation in rats
Sach-SW:Animal behavior
 Animal sociality
 Biological locomotion
 Burrowing
 Gene expression
 Neuronal plasticity
 Prefrontal cortex
 Rats
K10plus-PPN:1898444056
Verknüpfungen:→ Zeitschrift

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