Malaria parasite centrins can assemble by Ca2+-inducible condensation

• Verfasst von: Voss, Yannik [VerfasserIn]
• Verfasst von: Klaus, Severina [VerfasserIn]
• Verfasst von: Lichti, Nicolas [VerfasserIn]
• Verfasst von: Ganter, Markus [VerfasserIn]
• Verfasst von: Guizetti, Julien [VerfasserIn]
• Titel: Malaria parasite centrins can assemble by Ca2+-inducible condensation
• Verf.angabe: Yannik Voß, Severina Klaus, Nicolas P. Lichti, Markus Ganter, Julien Guizetti
• E-Jahr: 2023
• Jahr: Dezember 2023
• Umfang: 30 S.
• Fussnoten: Gesehen am 20.08.2024 ; Im Titel ist 2+ hochgestellt
• Titel Quelle: Enthalten in: Public Library of SciencePLoS pathogens
• Ort Quelle: Lawrence, Kan. : PLoS, 2005
• Jahr Quelle: 2023
• Band/Heft Quelle: 19(2023), 12 vom: Dez., Seite 1-30
• ISSN Quelle: 1553-7374
• DOI: doi:10.1371/journal.ppat.1011899
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: BINDING
• Sach-SW: CALCIUM
• Sach-SW: CELL-CYCLE
• Sach-SW: CENTROSOME
• Sach-SW: MICROTUBULES
• Sach-SW: PHOSPHORYLATION
• Sach-SW: PLASMODIUM-FALCIPARUM
• Sach-SW: PROTEIN SECONDARY STRUCTURE
• Sach-SW: SPINDLE POLE BODY
• Sach-SW: YEAST
• K10plus-PPN: 1899157425

Abstract

Centrins are small calcium-binding proteins that have a variety of roles and are universally associated with eukaryotic centrosomes. Rapid proliferation of the malaria-causing parasite Plasmodium falciparum in the human blood depends on a particularly divergent and acentriolar centrosome, which incorporates several essential centrins. Their precise mode of action, however, remains unclear. In this study calcium-inducible liquid-liquid phase separation is revealed as an evolutionarily conserved principle of assembly for multiple centrins from P. falciparum and other species. Furthermore, the disordered N-terminus and calcium-binding motifs are defined as essential features for reversible biomolecular condensation, and we demonstrate that certain centrins can form co-condensates. In vivo analysis using live cell STED microscopy shows liquid-like dynamics of centrosomal centrin. Additionally, implementation of an inducible protein overexpression system reveals concentration-dependent formation of extra-centrosomal centrin assemblies with condensate-like properties. The timing of foci formation and dissolution suggests that centrin assembly is regulated. This study thereby provides a new model for centrin accumulation at eukaryotic centrosomes.