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| Verfasst von: | Haubenreich, Carolin [VerfasserIn]  |
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| | Lenz, Michael [VerfasserIn] |
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| | Schuppert, Andreas [VerfasserIn] |
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| | Peitz, Michael [VerfasserIn] |
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| | Koch, Philipp [VerfasserIn] |
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| | Zenke, Martin [VerfasserIn] |
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| | Brüstle, Oliver [VerfasserIn] |
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| Titel: | Epigenetic and transcriptional shifts in human neural stem cells after reprogramming into induced pluripotent stem cells and subsequent redifferentiation |
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| Verf.angabe: | Carolin Haubenreich, Michael Lenz, Andreas Schuppert, Michael Peitz, Philipp Koch, Martin Zenke and Oliver Brüstle |
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| E-Jahr: | 2024 |
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| Jahr: | 12 March 2024 |
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| Umfang: | 15 S. |
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| Illustrationen: | Illustrationen |
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| Fussnoten: | Gesehen am 21.08.2024 |
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| Titel Quelle: | Enthalten in: International journal of molecular sciences |
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| Ort Quelle: | Basel : Molecular Diversity Preservation International, 2000 |
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| Jahr Quelle: | 2024 |
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| Band/Heft Quelle: | 25(2024), 6, Artikel-ID 3214, Seite 1-15 |
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| ISSN Quelle: | 1422-0067 |
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| | 1661-6596 |
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| Abstract: | Induced pluripotent stem cells (iPSCs) and their derivatives have been described to display epigenetic memory of their founder cells, as well as de novo reprogramming-associated alterations. In order to selectively explore changes due to the reprogramming process and not to heterologous somatic memory, we devised a circular reprogramming approach where somatic stem cells are used to generate iPSCs, which are subsequently re-differentiated into their original fate. As somatic founder cells, we employed human embryonic stem cell-derived neural stem cells (NSCs) and compared them to iPSC-derived NSCs derived thereof. Global transcription profiling of this isogenic circular system revealed remarkably similar transcriptomes of both NSC populations, with the exception of 36 transcripts. Amongst these we detected a disproportionately large fraction of X chromosomal genes, all of which were upregulated in iPSC-NSCs. Concurrently, we detected differential methylation of X chromosomal sites spatially coinciding with regions harboring differentially expressed genes. While our data point to a pronounced overall reinstallation of autosomal transcriptomic and methylation signatures when a defined somatic lineage is propagated through pluripotency, they also indicate that X chromosomal genes may partially escape this reinstallation process. Considering the broad application of iPSCs in disease modeling and regenerative approaches, such reprogramming-associated alterations in X chromosomal gene expression and DNA methylation deserve particular attention. |
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| DOI: | doi:10.3390/ijms25063214 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext: https://doi.org/10.3390/ijms25063214 |
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| | kostenfrei: Volltext: https://www.mdpi.com/1422-0067/25/6/3214 |
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| | DOI: https://doi.org/10.3390/ijms25063214 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | iPS cells |
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| | neural stem cells |
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| | pluripotency |
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| | reprogramming |
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| | X chromosome |
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| K10plus-PPN: | 1899282602 |
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| Verknüpfungen: | → Zeitschrift |
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Epigenetic and transcriptional shifts in human neural stem cells after reprogramming into induced pluripotent stem cells and subsequent redifferentiation / Haubenreich, Carolin [VerfasserIn]; 12 March 2024 (Online-Ressource)
