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| Verfasst von: | Yang, Guohuan [VerfasserIn]  |
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| | Schmid-Siegel, Maximilian [VerfasserIn] |
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| | Heissenberger, Clemens [VerfasserIn] |
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| | Koš-Braun, Isabelle C. [VerfasserIn] |
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| | Prechtl, Martina [VerfasserIn] |
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| | Meca-Laguna, Gabriel [VerfasserIn] |
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| | Rocha, Marta [VerfasserIn] |
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| | Wagner-Schrittwieser, Anja [VerfasserIn] |
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| | Pils, Vera [VerfasserIn] |
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| | Meixner, Barbara [VerfasserIn] |
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| | Tav, Koray [VerfasserIn] |
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| | Hengstschläger, Markus [VerfasserIn] |
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| | Grillari, Johannes [VerfasserIn] |
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| | Koš, Martin [VerfasserIn] |
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| | Schosserer, Markus [VerfasserIn] |
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| Titel: | 2′-O-ribose methylation levels of ribosomal RNA distinguish different types of growth arrest in human dermal fibroblasts |
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| Verf.angabe: | Guohuan Yang, Maximilian Schmid-Siegel, Clemens Heissenberger, Isabelle C. Kos-Braun, Martina Prechtl, Gabriel Meca-Laguna, Marta Rocha, Anja Wagner-Schrittwieser, Vera Pils, Barbara Meixner, Koray Tav, Markus Hengstschläger, Johannes Grillari, Martin Koš and Markus Schosserer |
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| E-Jahr: | 2024 |
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| Jahr: | February 2024 |
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| Umfang: | 10 S. |
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| Illustrationen: | Illustrationen |
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| Fussnoten: | Veröffentlicht: 12. Februar 2024 ; Gesehen am 21.08.2024 |
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| Titel Quelle: | Enthalten in: Journal of cell science |
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| Ort Quelle: | Cambridge : Company of Biologists Limited, 1853 |
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| Jahr Quelle: | 2024 |
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| Band/Heft Quelle: | 137(2024), 3 vom: Feb., Artikel-ID jcs261930, Seite 1-10 |
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| ISSN Quelle: | 1477-9137 |
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| Abstract: | The 2′-O-methylation (2′-O-Me) of ribosomal RNA (rRNA) shows plasticity that is potentially associated with cell phenotypes. We used RiboMeth-seq profiling to reveal growth arrest-specific 2′-O-Me patterns in primary human dermal fibroblasts from three different donors. We exposed cells to hydrogen peroxide to induce cellular senescence and to high cell densities to promote quiescence by contact inhibition. We compared both modes of cell cycle arrest to proliferating cells and could indeed distinguish these conditions by their overall 2′-O-Me patterns. Methylation levels at a small fraction of sites showed plasticity and correlated with the expression of specific small nucleolar RNAs (snoRNAs) but not with expression of fibrillarin. Moreover, we observed subtle senescence-associated alterations in ribosome biogenesis. Knockdown of the snoRNA SNORD87, which acts as a guide for modification of a hypermethylated position in non-proliferating cells, was sufficient to boost cell proliferation. Conversely, depletion of SNORD88A, SNORD88B and SNORD88C, which act as guides for modification of a hypomethylated site, caused decreased proliferation without affecting global protein synthesis or apoptosis. Taken together, our findings provide evidence that rRNA modifications can be used to distinguish and potentially influence specific growth phenotypes of primary cells. |
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| DOI: | doi:10.1242/jcs.261930 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext: https://doi.org/10.1242/jcs.261930 |
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| | DOI: https://doi.org/10.1242/jcs.261930 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 1899283722 |
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| Verknüpfungen: | → Zeitschrift |
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2′-O-ribose methylation levels of ribosomal RNA distinguish different types of growth arrest in human dermal fibroblasts / Yang, Guohuan [VerfasserIn]; February 2024 (Online-Ressource)
