Status: Bibliographieeintrag
Standort: ---
Exemplare:
---
| |  Online-Ressource |
|---|
| Verfasst von: | Quadt, Katharina [VerfasserIn]  |
|---|
| | Smyrnakou, Xanthoula [VerfasserIn] |
|---|
| | Frischknecht, Friedrich [VerfasserIn] |
|---|
| | Böse, Guido [VerfasserIn] |
|---|
| | Ganter, Markus [VerfasserIn] |
|---|
| Titel: | Plasmodium falciparum parasites exit the infected erythrocyte after haemolysis with saponin and streptolysin O |
|---|
| Verf.angabe: | Katharina A. Quadt, Xanthoula Smyrnakou, Friedrich Frischknecht, Guido Böse, Markus Ganter |
|---|
| Jahr: | 2020 |
|---|
| Umfang: | 6 S. |
|---|
| Illustrationen: | Illustrationen |
|---|
| Fussnoten: | Gesehen am 21.08.2024 |
|---|
| Titel Quelle: | Enthalten in: Parasitology research |
|---|
| Ort Quelle: | Berlin : Springer, 1928 |
|---|
| Jahr Quelle: | 2020 |
|---|
| Band/Heft Quelle: | 119(2020) vom: Dez., Seite 4297-4302 |
|---|
| ISSN Quelle: | 1432-1955 |
|---|
| Abstract: | Malaria is caused by unicellular parasites of the genus Plasmodium, which reside in erythrocytes during the clinically relevant stage of infection. To separate parasite from host cell material, haemolytic agents such as saponin are widely used. Previous electron microscopy studies on saponin-treated parasites reported both, parasites enclosed by the erythrocyte membrane and liberated from the host cell. These ambiguous reports prompted us to investigate haemolysis by live-cell time-lapse microscopy. Using either saponin or streptolysin O to lyse Plasmodium falciparum-infected erythrocytes, we found that ring-stage parasites efficiently exit the erythrocyte upon haemolysis. For late-stage parasites, we found that only approximately half were freed, supporting the previous electron microscopy studies. Immunofluorescence imaging indicated that freed parasites were surrounded by the parasitophorous vacuolar membrane. These results may be of interest for future work using haemolytic agents to enrich for parasite material. |
|---|
| DOI: | doi:10.1007/s00436-020-06932-9 |
|---|
| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1007/s00436-020-06932-9 |
|---|
| | Volltext: https://link.springer.com/article/10.1007/s00436-020-06932-9 |
|---|
| | DOI: https://doi.org/10.1007/s00436-020-06932-9 |
|---|
| Datenträger: | Online-Ressource |
|---|
| Sprache: | eng |
|---|
| Sach-SW: | Haemolysis |
|---|
| | Plasmodium falciparum |
|---|
| | Saponin |
|---|
| | Streptolysin O |
|---|
| K10plus-PPN: | 1899311610 |
|---|
| Verknüpfungen: | → Zeitschrift |
|---|
Plasmodium falciparum parasites exit the infected erythrocyte after haemolysis with saponin and streptolysin O / Quadt, Katharina [VerfasserIn]; 2020 (Online-Ressource)
69245975
