Status: Bibliographieeintrag
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| Verfasst von: | Salgania, Harpreet Kaur [VerfasserIn]  |
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| | Metz, Jutta [VerfasserIn] |
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| | Jeske, Mandy [VerfasserIn] |
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| Titel: | ReLo is a simple and rapid colocalization assay to identify and characterize direct protein-protein interactions |
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| Verf.angabe: | Harpreet Kaur Salgania, Jutta Metz & Mandy Jeske |
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| Jahr: | 2024 |
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| Umfang: | 12 S. |
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| Illustrationen: | Illustrationen |
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| Fussnoten: | Online veröffentlicht: 03 April 2024 ; Gesehen am 21.08.2024 |
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| Titel Quelle: | Enthalten in: Nature Communications |
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| Ort Quelle: | [London] : Springer Nature, 2010 |
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| Jahr Quelle: | 2024 |
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| Band/Heft Quelle: | 15(2024), Artikel-ID 2875, Seite 1-12 |
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| ISSN Quelle: | 2041-1723 |
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| Abstract: | The characterization of protein-protein interactions (PPIs) is fundamental to the understanding of biochemical processes. Many methods have been established to identify and study direct PPIs; however, screening and investigating PPIs involving large or poorly soluble proteins remains challenging. Here, we introduce ReLo, a simple, rapid, and versatile cell culture-based method for detecting and investigating interactions in a cellular context. Our experiments demonstrate that ReLo specifically detects direct binary PPIs. Furthermore, we show that ReLo bridging experiments can also be used to determine the binding topology of subunits within multiprotein complexes. In addition, ReLo facilitates the identification of protein domains that mediate complex formation, allows screening for interfering point mutations, and it is sensitive to drugs that mediate or disrupt an interaction. In summary, ReLo is a simple and rapid alternative for the study of PPIs, especially when studying structurally complex proteins or when established methods fail. |
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| DOI: | doi:10.1038/s41467-024-47233-4 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext: https://doi.org/10.1038/s41467-024-47233-4 |
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| | kostenfrei: Volltext: https://www.nature.com/articles/s41467-024-47233-4 |
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| | DOI: https://doi.org/10.1038/s41467-024-47233-4 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Cell culture |
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| | Protein-protein interaction networks |
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| | RNA-binding proteins |
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| | Two-hybrid system |
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| K10plus-PPN: | 1899314539 |
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| Verknüpfungen: | → Zeitschrift |
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ReLo is a simple and rapid colocalization assay to identify and characterize direct protein-protein interactions / Salgania, Harpreet Kaur [VerfasserIn]; 2024 (Online-Ressource)
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