Pretransplant CD4 helper function and interleukin 10 response predict risk of acute kidney graft rejection

• Verfasst von: Weimer, Rolf [VerfasserIn]
• Verfasst von: Zipperle, Silvia [VerfasserIn]
• Verfasst von: Daniel, Volker [VerfasserIn]
• Verfasst von: Carl, Stefan [VerfasserIn]
• Verfasst von: Staehler, Gerd [VerfasserIn]
• Verfasst von: Opelz, Gerhard [VerfasserIn]
• Titel: Pretransplant CD4 helper function and interleukin 10 response predict risk of acute kidney graft rejection
• Verf.angabe: Rolf Weimer, Silvia Zipperle, Volker Daniel, Stefan Carl, Gerd Staehler, Gerhard Opelz
• E-Jahr: 1996
• Jahr: December 15, 1996
• Umfang: 8 S.
• Fussnoten: Gesehen am 22.08.2024
• Titel Quelle: Enthalten in: Transplantation
• Ort Quelle: Hagerstown, Md. : Lippincott Williams & Wilkins, 1963
• Jahr Quelle: 1996
• Band/Heft Quelle: 62(1996), 11, Seite 1606-1614
• ISSN Quelle: 1534-6080
• DOI: doi:10.1097/00007890-199612150-00014
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1899350209

Abstract

In a prospective study of 80 patients, we investigated the association of acute kidney graft rejection with pretransplant T helper/suppressor activity, B-cell responses, and in vitro cytokine secretion. Patients' CD4+ or CD8+ T cells were cocultured with control B cells and pokeweed mitogen for 6 days. SAC I was used for T cell- and monocyte-independent B-cell stimulation and pokeweed mitogen was used for T cell-dependent B-cell stimulation. B-cell differentiation was assessed in a reverse hemolytic plaque assay. Cytokine responses of T cells (interleukin[IL]-2, IL-10, γ-interferon) and B cells/monocytes (IL-6, IL-8, tumor necrosis factor-α, granulocyte-macrophage colony-stimulating factor) were determined in culture supernatants using ELISA. Subsets of CD4+ T cells, CD8+ T cells, and B cells were assessed by flow cytometry None of 12 patients with pretransplant CD4 helper defects (CD4 helper activity<10%) had acute rejection episodes, in contrast to 32 of 68 (47%) patients with normal pretransplant CD4 helper function (P=0.001). Patients with pretransplant CD4 helper defects also had better 1-year graft function than patients without CD4 helper defects (serum creatinine 1.2±0.1 mg/dl and 1.7±0.1 mg/dl, respectively,P<0.05). Pretransplant IL-10 responses were significantly associated with the occurrence of acute rejection episodes(P=0.001) and impaired 1-year graft function(P<0.001). All 14 patients with low pretransplant IL-10 responses (<100 pg/ml) had 1-year serum creatinine values of <1.5 mg/dl. Pretransplant B-cell defects and B cell/monocyte-derived cytokine secretion were not related to the incidence of graft rejection or infectious complications. Pretransplant CD8 suppressor-effector (CD11b+) cell counts were significantly associated with the occurrence of infections(P<0.05). These results show that pretransplant CD4 helper defects and low IL-10 responses predict a low risk of graft rejection, whereas Th1 (IL-2, γ-interferon) and B-cell/monocyte responses are not of predictive value.