Status: Bibliographieeintrag
Standort: ---
Exemplare:
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| Online-Ressource |
Verfasst von: | Weimer, Rolf [VerfasserIn]  |
| Zipperle, Silvia [VerfasserIn]  |
| Daniel, Volker [VerfasserIn]  |
| Opelz, Gerhard [VerfasserIn]  |
Titel: | In vitro cytokine treatment of B cell defects in HIV-infected hemophilia patients |
Verf.angabe: | Rolf Weimer, Silvia Zipperle, Volker Daniel, Gerhard Opelz |
E-Jahr: | 1995 |
Jahr: | July 1995 |
Umfang: | 11 S. |
Fussnoten: | Gesehen am 22.08.2024 |
Titel Quelle: | Enthalten in: Vox sanguinis |
Ort Quelle: | Oxford [u.a.] : Wiley-Blackwell, 1956 |
Jahr Quelle: | 1995 |
Band/Heft Quelle: | 69(1995), 1, Seite 27-37 |
ISSN Quelle: | 1423-0410 |
Abstract: | HIV-infected patients exhibit defects in B cell differentiation and in the IL-6 response of B cells, in association with autoantibody formation against T cells. These autoantibodies have been implicated as important factors in the development of immunodeficiency disease. As the restoration of defective B cell responses might prevent autoantibody formation and the resulting immunosuppression, we studied whether in vitro treatment with recombinant IL-2 (rIL-2), recombinant IL-4 (rIL-4) or recombinant IL-6 (rIL-6) might restore the response of B cells of HIV-infected patients. B cells of 6 HIV-negative hemophilia patients, 4 HIV-positive patients at CDC stage II, III, 4 HIV-positive patients at CDC stage IV, and 6 healthy controls were tested in Staphylococcus aureus Cowan I (SAC-I)-stimulated B cell cultures and Pokeweed mitogen (PWM)-stimulated allogeneic B and T cell cocultures. B cell differentiation was assessed in a reverse hemolytic plaque assay and by ELISA determination of IgM, IgG and IL-6 in culture supernatants. In vitro application of rIL-6 resulted in suppression of both elevated unstimulated and mitogen-stimulated B cell responses in a dose-dependent manner which was in part due to feedback inhibition. PWM- and SAC-I-stimulated IgG and IgM responses, respectively, could be restored after addition of 10 U/ml rIL-2 in HIV-negative patients, but not in HIV-positive patients. Addition of rIL-4 to cultures resulted in suppression of both unstimulated and mitogen-stimulated IL-6 secretion and B cell responses. Severely depressed B cell responses in CDC IV patients were not significantly affected by cytokine application. These results indicate that defective Ig responses in HIV-negative patients may be restored by rIL-2 treatment whereas HIV-induced B cell defects are not corrected by supply of T cell help or cytokines promoting B cell growth and differentiation. |
DOI: | doi:10.1111/j.1423-0410.1995.tb00344.x |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1111/j.1423-0410.1995.tb00344.x |
| Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1423-0410.1995.tb00344.x |
| DOI: https://doi.org/10.1111/j.1423-0410.1995.tb00344.x |
Datenträger: | Online-Ressource |
Sprache: | eng |
K10plus-PPN: | 1899376739 |
Verknüpfungen: | → Zeitschrift |
In vitro cytokine treatment of B cell defects in HIV-infected hemophilia patients / Weimer, Rolf [VerfasserIn]; July 1995 (Online-Ressource)
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