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Status: Bibliographieeintrag

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Verfasst von:Guo, Feng [VerfasserIn]   i
 Kan, Kejia [VerfasserIn]   i
 Rückert, Felix [VerfasserIn]   i
 Rückert, Wolfgang [VerfasserIn]   i
 Li, Lin [VerfasserIn]   i
 Eberhard, Johannes [VerfasserIn]   i
 May, Tobias [VerfasserIn]   i
 Sticht, Carsten [VerfasserIn]   i
 Dirks, Wilhelm G. [VerfasserIn]   i
 Reißfelder, Christoph [VerfasserIn]   i
 Pallavi, Prama [VerfasserIn]   i
 Keese, Michael [VerfasserIn]   i
Titel:Comparison of tumour-specific phenotypes in human primary and expandable pancreatic cancer cell lines
Verf.angabe:Feng Guo, Kejia Kan, Felix Rückert, Wolfgang Rückert, Lin Li, Johannes Eberhard, Tobias May, Carsten Sticht, Wilhelm G. Dirks, Christoph Reißfelder, Prama Pallavi and Michael Keese
E-Jahr:2023
Jahr:31 August 2023
Umfang:22 S.
Illustrationen:Illustrationen
Fussnoten:Gesehen am 02.09.2024
Titel Quelle:Enthalten in: International journal of molecular sciences
Ort Quelle:Basel : Molecular Diversity Preservation International, 2000
Jahr Quelle:2023
Band/Heft Quelle:24(2023), 17, Artikel-ID 13530, Seite 1-22
ISSN Quelle:1422-0067
 1661-6596
Abstract:There is an ongoing need for patient-specific chemotherapy for pancreatic cancer. Tumour cells isolated from human tissues can be used to predict patients’ response to chemotherapy. However, the isolation and maintenance of pancreatic cancer cells is challenging because these cells become highly vulnerable after losing the tumour microenvironment. Therefore, we investigated whether the cells retained their original characteristics after lentiviral transfection and expansion. Three human primary pancreatic cancer cell lines were lentivirally transduced to create expandable (Ex) cells which were then compared with primary (Pri) cells. No obvious differences in the morphology or epithelial-mesenchymal transition (EMT) were observed between the primary and expandable cell lines. The two expandable cell lines showed higher proliferation rates in the 2D and 3D models. All three expandable cell lines showed attenuated migratory ability. Differences in gene expression between primary and expandable cell lines were then compared using RNA-Seq data. Potential target drugs were predicted by differentially expressed genes (DEGs), and differentially expressed pathways (DEPs) related to tumour-specific characteristics such as proliferation, migration, EMT, drug resistance, and reactive oxygen species (ROS) were investigated using the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. We found that the two expandable cell lines expressed similar chemosensitivity and redox-regulatory capability to gemcitabine and oxaliplatin in the 2D model as compared to their counterparts. In conclusion, we successfully generated expandable primary pancreatic cancer cell lines using lentiviral transduction. These expandable cells not only retain some tumour-specific biological traits of primary cells but also show an ongoing proliferative capacity, thereby yielding sufficient material for drug response assays, which may provide a patient-specific platform for chemotherapy drug screening.
DOI:doi:10.3390/ijms241713530
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

kostenfrei: Volltext: https://doi.org/10.3390/ijms241713530
 kostenfrei: Volltext: https://www.mdpi.com/1422-0067/24/17/13530
 DOI: https://doi.org/10.3390/ijms241713530
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:chemosensitivity
 chemotherapy
 patient-tailored therapy
 regression analysis
 RNA-Seq
 statistical comparison of populations
K10plus-PPN:1900841371
Verknüpfungen:→ Zeitschrift

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