Differential expression of APO-1 on human thymocytes

• Verfasst von: Debatin, Klaus-Michael [VerfasserIn]
• Verfasst von: Süss, Dorothee [VerfasserIn]
• Verfasst von: Krammer, Peter H. [VerfasserIn]
• Titel: Differential expression of APO-1 on human thymocytes
• Titelzusatz: implications for negative selection?
• Verf.angabe: Klaus-Michael Debatin, Dorothee Süss, Peter H. Krammer
• E-Jahr: 1994
• Jahr: March 1994
• Umfang: 6 S.
• Fussnoten: Elektronische Reproduktion der Druck-Ausgabe 23. November 2005 ; Gesehen am 24.09.2024
• Titel Quelle: Enthalten in: European journal of immunology
• Ort Quelle: Weinheim : Wiley-VCH, 1971
• Jahr Quelle: 1994
• Band/Heft Quelle: 24(1994), 3, Seite 753-758
• ISSN Quelle: 1521-4141
• DOI: doi:10.1002/eji.1830240339
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Apoptosis
• Sach-SW: Negative selection
• Sach-SW: Thymocyte subpopulations
• K10plus-PPN: 1903350069

Abstract

Negative selection during T cell ontogeny involves selective induction of apoptosis in thymocytes. In peripheral lymphoid cells, apoptosis may be mediated via the APO-1 pathway. Here we report that APO-1 is constitutively expressed on the vast majority of human thymocytes but down-regulated at a mature stage of thymocyte development (TCRhi). This stage of development is characterized by CD28hi, CD44hi, CD69hi and up-regulation of Bcl-2 protein. We define a new thymocyte subpopulation that expresses high levels of APO-1 and intermediate levels of T cell receptor α/β (TCRim/APO-1hi). The TCRim/APO-1hi population contains a large fraction of dead cells, suggesting that the APO-1 pathway may be involved in negative selection of at least a fraction of thymocytes after intrathymic activation.