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Verfasst von:Schlautmann, Lena P. [VerfasserIn]   i
 Lackmann, Jan-Wilm [VerfasserIn]   i
 Altmüller, Janine [VerfasserIn]   i
 Dieterich, Christoph [VerfasserIn]   i
 Böhm, Volker [VerfasserIn]   i
 Gehring, Niels H. [VerfasserIn]   i
Titel:Exon junction complex-associated multi-adapter RNPS1 nucleates splicing regulatory complexes to maintain transcriptome surveillance
Verf.angabe:Lena P. Schlautmann, Jan-Wilm Lackmann, Janine Altmüller, Christoph Dieterich, Volker Boehm and Niels H. Gehring
E-Jahr:2022
Jahr:30 May 2022
Umfang:20 S.
Illustrationen:Illustrationen
Fussnoten:Gesehen am 25.09.2024
Titel Quelle:Enthalten in: Nucleic acids research
Ort Quelle:Oxford : Oxford Univ. Press, 1974
Jahr Quelle:2022
Band/Heft Quelle:50(2022), 10, Seite 5899-5918
ISSN Quelle:1362-4962
Abstract:The exon junction complex (EJC) is an RNA-binding multi-protein complex with critical functions in post-transcriptional gene regulation. It is deposited on the mRNA during splicing and regulates diverse processes including pre-mRNA splicing and nonsense-mediated mRNA decay (NMD) via various interacting proteins. The peripheral EJC-binding protein RNPS1 was reported to serve two insufficiently characterized functions: suppressing mis-splicing of cryptic splice sites and activating NMD in the cytoplasm. The analysis of transcriptome-wide effects of EJC and RNPS1 knockdowns in different human cell lines supports the conclusion that RNPS1 can moderately influence NMD activity, but is not a globally essential NMD factor. However, numerous aberrant splicing events strongly suggest that the main function of RNPS1 is splicing regulation. Rescue analyses revealed that the RRM and C-terminal domain of RNPS1 both contribute partially to regulate RNPS1-dependent splicing events. We defined the RNPS1 core interactome using complementary immunoprecipitations and proximity labeling, which identified interactions with splicing-regulatory factors that are dependent on the C-terminus or the RRM domain of RNPS1. Thus, RNPS1 emerges as a multifunctional splicing regulator that promotes correct and efficient splicing of different vulnerable splicing events via the formation of diverse splicing-promoting complexes.
DOI:doi:10.1093/nar/gkac428
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

kostenfrei: Volltext: https://doi.org/10.1093/nar/gkac428
 kostenfrei: Volltext: https://academic.oup.com/nar/article/50/10/5899/6595294
 DOI: https://doi.org/10.1093/nar/gkac428
Datenträger:Online-Ressource
Sprache:eng
K10plus-PPN:1903448867
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