Status: Bibliographieeintrag
Standort: ---
Exemplare:
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| Online-Ressource |
Verfasst von: | Schlautmann, Lena P. [VerfasserIn]  |
| Lackmann, Jan-Wilm [VerfasserIn]  |
| Altmüller, Janine [VerfasserIn]  |
| Dieterich, Christoph [VerfasserIn]  |
| Böhm, Volker [VerfasserIn]  |
| Gehring, Niels H. [VerfasserIn]  |
Titel: | Exon junction complex-associated multi-adapter RNPS1 nucleates splicing regulatory complexes to maintain transcriptome surveillance |
Verf.angabe: | Lena P. Schlautmann, Jan-Wilm Lackmann, Janine Altmüller, Christoph Dieterich, Volker Boehm and Niels H. Gehring |
E-Jahr: | 2022 |
Jahr: | 30 May 2022 |
Umfang: | 20 S. |
Illustrationen: | Illustrationen |
Fussnoten: | Gesehen am 25.09.2024 |
Titel Quelle: | Enthalten in: Nucleic acids research |
Ort Quelle: | Oxford : Oxford Univ. Press, 1974 |
Jahr Quelle: | 2022 |
Band/Heft Quelle: | 50(2022), 10, Seite 5899-5918 |
ISSN Quelle: | 1362-4962 |
Abstract: | The exon junction complex (EJC) is an RNA-binding multi-protein complex with critical functions in post-transcriptional gene regulation. It is deposited on the mRNA during splicing and regulates diverse processes including pre-mRNA splicing and nonsense-mediated mRNA decay (NMD) via various interacting proteins. The peripheral EJC-binding protein RNPS1 was reported to serve two insufficiently characterized functions: suppressing mis-splicing of cryptic splice sites and activating NMD in the cytoplasm. The analysis of transcriptome-wide effects of EJC and RNPS1 knockdowns in different human cell lines supports the conclusion that RNPS1 can moderately influence NMD activity, but is not a globally essential NMD factor. However, numerous aberrant splicing events strongly suggest that the main function of RNPS1 is splicing regulation. Rescue analyses revealed that the RRM and C-terminal domain of RNPS1 both contribute partially to regulate RNPS1-dependent splicing events. We defined the RNPS1 core interactome using complementary immunoprecipitations and proximity labeling, which identified interactions with splicing-regulatory factors that are dependent on the C-terminus or the RRM domain of RNPS1. Thus, RNPS1 emerges as a multifunctional splicing regulator that promotes correct and efficient splicing of different vulnerable splicing events via the formation of diverse splicing-promoting complexes. |
DOI: | doi:10.1093/nar/gkac428 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext: https://doi.org/10.1093/nar/gkac428 |
| kostenfrei: Volltext: https://academic.oup.com/nar/article/50/10/5899/6595294 |
| DOI: https://doi.org/10.1093/nar/gkac428 |
Datenträger: | Online-Ressource |
Sprache: | eng |
K10plus-PPN: | 1903448867 |
Verknüpfungen: | → Zeitschrift |
Exon junction complex-associated multi-adapter RNPS1 nucleates splicing regulatory complexes to maintain transcriptome surveillance / Schlautmann, Lena P. [VerfasserIn]; 30 May 2022 (Online-Ressource)
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