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Verfasst von:Ghasemi, Nima [VerfasserIn]   i
 Azizi, Hossein [VerfasserIn]   i
 Skutella, Thomas [VerfasserIn]   i
Titel:Unraveling the significance of nanog in the generation of embryonic stem-like cells from spermatogonia stem cells
Titelzusatz:a combined in silico analysis and in vitro experimental approach
Verf.angabe:Nima Ghasemi, Hossein Azizi, Thomas Skutella
E-Jahr:2024
Jahr:29 April 2024
Umfang:16 S.
Fussnoten:Gesehen am 30.09.2024
Titel Quelle:Enthalten in: International journal of molecular sciences
Ort Quelle:Basel : Molecular Diversity Preservation International, 2000
Jahr Quelle:2024
Band/Heft Quelle:25(2024), 9, Artikel-ID 4833, Seite 4833-1-4833-16
ISSN Quelle:1422-0067
 1661-6596
Abstract:Embryonic stem-like cells (ES-like cells) are promising for medical research and clinical applications. Traditional methods involve "Yamanaka" transcription (OSKM) to derive these cells from somatic cells in vitro. Recently, a novel approach has emerged, obtaining ES-like cells from spermatogonia stem cells (SSCs) in a time-related process without adding artificial additives to cell cultures, like transcription factors or small molecules such as pten or p53 inhibitors. This study aims to investigate the role of the Nanog in the conversion of SSCs to pluripotent stem cells through both in silico analysis and in vitro experiments. We used bioinformatic methods and microarray data to find significant genes connected to this derivation path, to construct PPI networks, using enrichment analysis, and to construct miRNA-lncRNA networks, as well as in vitro experiments, immunostaining, and Fluidigm qPCR analysis to connect the dots of Nanog significance. We concluded that Nanog is one of the most crucial differentially expressed genes during SSC conversion, collaborating with critical regulators such as Sox2, Dazl, Pou5f1, Dnmt3, and Cdh1. This intricate protein network positions Nanog as a pivotal factor in pathway enrichment for generating ES-like cells, including Wnt signaling, focal adhesion, and PI3K-Akt-mTOR signaling. Nanog expression is presumed to play a vital role in deriving ES-like cells from SSCs in vitro. Finding its pivotal role in this path illuminates future research and clinical applications.
DOI:doi:10.3390/ijms25094833
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.3390/ijms25094833
 Volltext: https://www.mdpi.com/1422-0067/25/9/4833
 DOI: https://doi.org/10.3390/ijms25094833
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:ES-like cells
 immunostaining
 Nanog
 pluripotency
 PPI networks
 spermatogonia stem cells
K10plus-PPN:1903712114
Verknüpfungen:→ Zeitschrift

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