| |  Online-Ressource |
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| Verfasst von: | Kamal, Shady Mansour [VerfasserIn]  |
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| | Cimdins-Ahne, Annika [VerfasserIn] |
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| | Lee, Changhan [VerfasserIn] |
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| | Li, Fengyang [VerfasserIn] |
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| | Martín-Rodríguez, Alberto J. [VerfasserIn] |
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| | Seferbekova, Zaira [VerfasserIn] |
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| | Afasizhev, Robert [VerfasserIn] |
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| | Wami, Haleluya Tesfaye [VerfasserIn] |
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| | Katikaridis, Panagiotis [VerfasserIn] |
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| | Meins, Lena [VerfasserIn] |
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| | Lünsdorf, Heinrich [VerfasserIn] |
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| | Dobrindt, Ulrich [VerfasserIn] |
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| | Mogk, Axel [VerfasserIn] |
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| | Römling, Ute [VerfasserIn] |
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| Titel: | A recently isolated human commensal Escherichia coli ST10 clone member mediates enhanced thermotolerance and tetrathionate respiration on a P1 phage-derived IncY plasmid |
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| Verf.angabe: | Shady Mansour Kamal, Annika Cimdins-Ahne, Changhan Lee, Fengyang Li, Alberto J. Martín-Rodríguez, Zaira Seferbekova, Robert Afasizhev, Haleluya Tesfaye Wami, Panagiotis Katikaridis, Lena Meins, Heinrich Lünsdorf, Ulrich Dobrindt, Axel Mogk, Ute Römling |
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| Jahr: | 2021 |
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| Umfang: | 17 S. |
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| Illustrationen: | Illustrationen |
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| Fussnoten: | "First published: 28 September 2020".- Artikel-Front door ; Gesehen am 07.10.2024 |
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| Titel Quelle: | Enthalten in: Molecular microbiology |
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| Ort Quelle: | Oxford [u.a.] : Wiley-Blackwell, 1987 |
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| Jahr Quelle: | 2021 |
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| Band/Heft Quelle: | 115(2021), 2, Seite 255-271 |
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| ISSN Quelle: | 1365-2958 |
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| Abstract: | The ubiquitous human commensal Escherichia coli has been well investigated through its model representative E. coli K-12. In this work, we initially characterized E. coli Fec10, a recently isolated human commensal strain of phylogroup A/sequence type ST10. Compared to E. coli K-12, the 4.88 Mbp Fec10 genome is characterized by distinct single-nucleotide polymorphisms and acquisition of genomic islands. In addition, E. coli Fec10 possesses a 155.86 kbp IncY plasmid, a composite element based on phage P1. pFec10 harbours multiple cargo genes such as coding for a tetrathionate reductase and its corresponding regulatory two-component system. Among the cargo genes is also the Transmissible Locus of Protein Quality Control (TLPQC), which mediates tolerance to lethal temperatures in bacteria. The disaggregase ClpGGI of TLPQC constitutes a major determinant of the thermotolerance of E. coli Fec10. We confirmed stand-alone disaggregation activity, but observed distinct biochemical characteristics of ClpGGI-Fec10 compared to the nearly identical Pseudomonas aeruginosa ClpGGI-SG17M. Furthermore, we noted a unique contribution of ClpGGI-Fec10 to the exquisite thermotolerance of E. coli Fec10, suggesting functional differences between both disaggregases in vivo. Detection of thermotolerance in 10% of human commensal E. coli isolates hints to the successful establishment of food-borne heat-resistant strains in the human gut. |
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| DOI: | doi:10.1111/mmi.14614 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext: https://doi.org/10.1111/mmi.14614 |
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| | kostenfrei: Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1111/mmi.14614 |
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| | DOI: https://doi.org/10.1111/mmi.14614 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | disaggregase ClpG |
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| | Escherichia coli |
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| | IncY plasmid |
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| | phylogenetic analysis |
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| | tetrathionate respiration |
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| | thermotolerance |
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| K10plus-PPN: | 1904977774 |
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| Verknüpfungen: | → Zeitschrift |
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¬A¬ recently isolated human commensal Escherichia coli ST10 clone member mediates enhanced thermotolerance and tetrathionate respiration on a P1 phage-derived IncY plasmid / Kamal, Shady Mansour [VerfasserIn]; 2021 (Online-Ressource)
