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Verfasst von:Lindner, Christina [VerfasserIn]   i
 Friemel, Anke [VerfasserIn]   i
 Schwegler, Niklas [VerfasserIn]   i
 Timmermann, Lisa [VerfasserIn]   i
 Pham, Truc Lam [VerfasserIn]   i
 Reusche, Vanessa [VerfasserIn]   i
 Kovermann, Michael [VerfasserIn]   i
 Thomas, Franziska [VerfasserIn]   i
Titel:Thermostable WW-domain scaffold to design functional β-sheet miniproteins
Verf.angabe:Christina Lindner, Anke Friemel, Niklas Schwegler, Lisa Timmermann, Truc Lam Pham, Vanessa Reusche, Michael Kovermann, and Franziska Thomas
E-Jahr:2024
Jahr:June 10, 2024
Umfang:11 S.
Illustrationen:Illustrationen
Fussnoten:Gesehen am 14.10.2024
Titel Quelle:Enthalten in: American Chemical SocietyJournal of the American Chemical Society
Ort Quelle:Washington, DC : ACS Publications, 1879
Jahr Quelle:2024
Band/Heft Quelle:146(2024), 24, Seite 16590-16600
ISSN Quelle:1520-5126
Abstract:There has been a recent surge in the design of miniproteins for medicinal chemistry, biomaterial design, or synthetic biology. In particular, there is an interest in peptide scaffolds that fold reliably, predictably, and with solid stability. In this article, we present the design of a highly thermostable WW domain, a three-stranded β-sheet motif, with a superior melting temperature of about 90 °C to serve as a core scaffold onto which receptor-like properties can be grafted. We have performed specific rounds of sequence iteration on a WW-domain consensus sequence to decipher sequence positions that affect structural and, thus, thermal stability. We identified a sequence-structure relationship that yields a highly thermostable WW-domain scaffold. High-resolution NMR spectroscopy was applied, which enabled the identification of structural features at the atomic scale that contribute to this high thermostability. Finally, we grafted the binding motifs of the three WW-domain groups─Group I, Group II/III, and Group IV─and organophosphate and metal binding onto the highly thermostable WW-domain scaffold and obtained thermostable de novo WW domains that indeed display the different binding modes that were intended. The organophosphate-binding WW domains exhibit melting temperatures that are up to 34 K higher than previously reported top-down designs. These results impressively demonstrate that the highly thermostable WW-domain core scaffold is a solid platform for the design of discrete and reliably folding functional β-sheet peptide miniproteins, providing an essential addition to the toolbox of peptide scaffolds previously used in synthetic biology and material design.
DOI:doi:10.1021/jacs.4c03498
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.1021/jacs.4c03498
 DOI: https://doi.org/10.1021/jacs.4c03498
Datenträger:Online-Ressource
Sprache:eng
K10plus-PPN:1905549822
Verknüpfungen:→ Zeitschrift

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