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Verfasst von:Pan, Qin [VerfasserIn]   i
 Mercker, Moritz [VerfasserIn]   i
 Klimovich, Alexander [VerfasserIn]   i
 Wittlieb, Jörg [VerfasserIn]   i
 Marciniak-Czochra, Anna [VerfasserIn]   i
 Böttger, Angelika [VerfasserIn]   i
Titel:Genetic interference with HvNotch provides new insights into the role of the Notch-signalling pathway for developmental pattern formation in Hydra
Verf.angabe:Qin Pan, Moritz Mercker, Alexander Klimovich, Jörg Wittlieb, Anna Marciniak-Czochra & Angelika Böttger
E-Jahr:2024
Jahr:12 April 2024
Umfang:16 S.
Illustrationen:Illustrationen
Fussnoten:Gesehen am 14.10.2024
Titel Quelle:Enthalten in: Scientific reports
Ort Quelle:[London] : Springer Nature, 2011
Jahr Quelle:2024
Band/Heft Quelle:14(2024), Artikel-ID 8553, Seite 1-16
ISSN Quelle:2045-2322
Abstract:The Notch-signalling pathway plays an important role in pattern formation in Hydra. Using pharmacological Notch inhibitors (DAPT and SAHM1), it has been demonstrated that HvNotch is required for head regeneration and tentacle patterning in Hydra. HvNotch is also involved in establishing the parent-bud boundary and instructing buds to develop feet and detach from the parent. To further investigate the functions of HvNotch, we successfully constructed NICD (HvNotch intracellular domain)-overexpressing and HvNotch-knockdown transgenic Hydra strains. NICD-overexpressing transgenic Hydra showed a pronounced inhibition on the expression of predicted HvNotch-target genes, suggesting a dominant negative effect of ectopic NICD. This resulted in a “Y-shaped” phenotype, which arises from the parent-bud boundary defect seen in polyps treated with DAPT. Additionally, “multiple heads”, “two-headed” and “ectopic tentacles” phenotypes were observed. The HvNotch-knockdown transgenic Hydra with reduced expression of HvNotch exhibited similar, but not identical phenotypes, with the addition of a “two feet” phenotype. Furthermore, we observed regeneration defects in both, overexpression and knockdown strains. We integrated these findings into a mathematical model based on long-range gradients of signalling molecules underlying sharply defined positions of HvNotch-signalling cells at the Hydra tentacle and bud boundaries.
DOI:doi:10.1038/s41598-024-58837-7
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

kostenfrei: Volltext: https://doi.org/10.1038/s41598-024-58837-7
 kostenfrei: Volltext: https://www.nature.com/articles/s41598-024-58837-7
 DOI: https://doi.org/10.1038/s41598-024-58837-7
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Cell biology
 Pattern formation
K10plus-PPN:1905618247
Verknüpfungen:→ Zeitschrift

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