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| Verfasst von: | Chi, Huanting [VerfasserIn]  |
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| | Qu, Bingqian [VerfasserIn] |
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| | Prawira, Angga [VerfasserIn] |
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| | Richardt, Talisa [VerfasserIn] |
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| | Maurer, Lars [VerfasserIn] |
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| | Hu, Jungen [VerfasserIn] |
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| | Fu, Rebecca Menhua [VerfasserIn] |
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| | Lempp, Florian A. [VerfasserIn] |
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| | Zhang, Zhenfeng [VerfasserIn] |
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| | Grimm, Dirk [VerfasserIn] |
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| | Wu, Xianfang [VerfasserIn] |
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| | Urban, Stephan [VerfasserIn] |
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| | Dao Thi, Viet Loan [VerfasserIn] |
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| Titel: | An hepatitis B and D virus infection model using human pluripotent stem cell-derived hepatocytes |
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| Verf.angabe: | Huanting Chi, Bingqian Qu, Angga Prawira, Talisa Richardt, Lars Maurer, Jungen Hu, Rebecca M Fu, Florian A Lempp, Zhenfeng Zhang, Dirk Grimm, Xianfang Wu, Stephan Urban & Viet Loan Dao Thi |
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| E-Jahr: | 2024 |
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| Jahr: | October 10, 2024 |
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| Umfang: | 26 S. |
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| Fussnoten: | Online: 04. September 2024 ; Gesehen am 17.10.2024 |
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| Titel Quelle: | Enthalten in: European Molecular Biology OrganizationEMBO reports |
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| Ort Quelle: | [London] : Nature Publishing Group UK, 2000 |
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| Jahr Quelle: | 2024 |
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| Band/Heft Quelle: | 25(2024), 10 vom: Okt., Seite 4311-4336 |
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| ISSN Quelle: | 1469-3178 |
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| Abstract: | Current culture systems available for studying hepatitis D virus (HDV) are suboptimal. In this study, we demonstrate that hepatocyte-like cells (HLCs) derived from human pluripotent stem cells (hPSCs) are fully permissive to HDV infection across various tested genotypes. When co-infected with the helper hepatitis B virus (HBV) or transduced to express the HBV envelope protein HBsAg, HLCs effectively release infectious progeny virions. We also show that HBsAg-expressing HLCs support the extracellular spread of HDV, thus providing a valuable platform for testing available anti-HDV regimens. By challenging the cells along the differentiation with HDV infection, we have identified CD63 as a potential HDV co-entry factor that was rate-limiting for HDV infection in immature hepatocytes. Given their renewable source and the potential to derive hPSCs from individual patients, we propose HLCs as a promising model for investigating HDV biology. Our findings offer new insights into HDV infection and expand the repertoire of research tools available for the development of therapeutic interventions. |
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| DOI: | doi:10.1038/s44319-024-00236-0 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext: https://doi.org/10.1038/s44319-024-00236-0 |
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| | kostenfrei: Volltext: https://www.embopress.org/doi/full/10.1038/s44319-024-00236-0 |
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| | DOI: https://doi.org/10.1038/s44319-024-00236-0 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Antiviral Treatment |
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| | Hepatitis B Virus |
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| | Hepatitis D Virus |
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| | Hepatocyte-like Cells |
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| | Human Pluripotent Stem Cells |
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| K10plus-PPN: | 1905961545 |
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| Verknüpfungen: | → Zeitschrift |
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¬An¬ hepatitis B and D virus infection model using human pluripotent stem cell-derived hepatocytes / Chi, Huanting [VerfasserIn]; October 10, 2024 (Online-Ressource)
