Resistance of chimpanzee T cells to human immunodeficiency virus type 1 Tat-enhanced oxidative stress and apoptosis

• Verfasst von: Ehret, Andreas [VerfasserIn]
• Verfasst von: Westendorp, Michael [VerfasserIn]
• Verfasst von: Herr, Ingrid [VerfasserIn]
• Verfasst von: Debatin, Klaus-Michael [VerfasserIn]
• Verfasst von: Heeney, Jonathan L. [VerfasserIn]
• Verfasst von: Frank, Rainer [VerfasserIn]
• Verfasst von: Krammer, Peter H. [VerfasserIn]
• Titel: Resistance of chimpanzee T cells to human immunodeficiency virus type 1 Tat-enhanced oxidative stress and apoptosis
• Verf.angabe: Andreas Ehret, Michael O. Westendorp, Ingrid Herr, Klaus-Michael Debatin, Jonathan L. Heeney, Rainer Frank, Peter H. Krammer
• E-Jahr: 1996
• Jahr: September 1, 1996
• Umfang: 6 S.
• Fussnoten: Gesehen am 21.10.2024
• Titel Quelle: Enthalten in: Journal of virology
• Ort Quelle: Baltimore, Md. : Soc., 1967
• Jahr Quelle: 1996
• Band/Heft Quelle: 70(1996), 9, Seite 6502-6507
• ISSN Quelle: 1098-5514
• DOI: doi:10.1128/jvi.70.9.6502-6507.1996
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1906330360

Abstract

CD4+ T-cell depletion in AIDS patients involves induction of apoptosis in human immunodeficiency virus (HIV)-infected and noninfected T cells. The HIV type 1 (HIV-1)-transactivating protein Tat enhances apoptosis and activation-induced cell death (AICD) of human T cells. This effect is mediated by the CD95 (APO-1/Fas) receptor-CD95 ligand (CD95L) system and may be linked to the induction of oxidative stress by Tat. Here we show that HIV-1 Tat-induced oxidative stress is necessary for sensitized AICD in T cells caused by CD95L expression. Tat-enhanced apoptosis and CD95L expression in T cells are inhibited by neutralizing anti-Tat antibodies, antioxidants, and the Tat inhibitor Ro24-7429. Chimpanzees infected with HIV-1 show viral replication resembling early infection in humans but do not show T-cell depletion or progression towards AIDS. The cause for this discrepancy is unknown. Here we show that unlike Tat-treated T cells in humans, Tat-treated chimpanzee T cells do not show downregulation of manganese superoxide dismutase or signs of oxidative stress. Chimpanzee T cells are also resistant to Tat-enhanced apoptosis, AICD, and CD95L upregulation.