| Online-Ressource |
Verfasst von: | Rühl, Heiko [VerfasserIn]  |
| Bode, Christian [VerfasserIn]  |
| Becher, Tobias [VerfasserIn]  |
| Eckert, Sebastian [VerfasserIn]  |
| Mohsen, Ghaith [VerfasserIn]  |
| McRae, Hannah L. [VerfasserIn]  |
| Müller, Jens [VerfasserIn]  |
| Reda, Sara [VerfasserIn]  |
| Loßnitzer, Dirk [VerfasserIn]  |
| Oldenburg, Johannes [VerfasserIn]  |
| Putensen, Christian [VerfasserIn]  |
| Pötzsch, Bernd [VerfasserIn]  |
Titel: | Decreased protein C pathway activity in COVID-19 compared to non-COVID sepsis |
Titelzusatz: | an observational and comparative cohort study |
Verf.angabe: | Heiko Rühl, Christian Bode, Tobias Becher, Sebastian Eckert, Ghaith Mohsen, Hannah L. McRae, Jens Müller, Sara Reda, Dirk Loßnitzer, Johannes Oldenburg, Christian Putensen and Bernd Pötzsch |
E-Jahr: | 2024 |
Jahr: | 2 September 2024 |
Umfang: | 12 S. |
Illustrationen: | Illustrationen |
Fussnoten: | Gesehen am 23.10.2024 |
Titel Quelle: | Enthalten in: Biomedicines |
Ort Quelle: | Basel : MDPI, 2013 |
Jahr Quelle: | 2024 |
Band/Heft Quelle: | 12(2024), 9, Artikel-ID 1982, Seite 1-12 |
ISSN Quelle: | 2227-9059 |
Abstract: | Sepsis-associated coagulopathy increases risk of mortality. Impairment of the anticoagulant protein C (PC) pathway may contribute to the thrombotic phenotype in coronavirus disease 2019 (COVID-19) sepsis. This study assessed the functionality of this pathway in COVID-19 and non-COVID sepsis by measuring its key enzymes, thrombin and activated PC (APC). The study population included 30 patients with COVID-19, 47 patients with non-COVID sepsis, and 40 healthy controls. In healthy controls, coagulation activation and subsequent APC formation was induced by 15 µg/kg recombinant activated factor VII one hour before blood sampling. APC and thrombin in plasma were measured using oligonucleotide-based enzyme capture assays. The indirect thrombin markers prothrombin-fragment 1+2 (F1+2) and thrombin-antithrombin complex (TAT) were also measured. Compared with stimulated healthy controls, median thrombin, F1+2, and TAT levels were higher in patients with COVID-19 (up to 6-fold, p < 2 × 10−6) and non-COVID sepsis (up to 4.7-fold, p < 0.010). APC levels were 2.4-fold higher in patients with COVID-19 (7.44 pmol/L, p = 0.011) and 3.4-fold higher in non-COVID sepsis patients (10.45 pmol/L, p = 2 × 10−4) than in controls (3.08 pmol/L). Thrombin markers and APC showed correlation in both COVID-19 (r = 0.364-0.661) and non-COVID sepsis patients (r = 0.535-0.711). After adjustment for PC levels, median APC/thrombin, APC/F1+2, and APC/TAT ratios were 2-fold (p = 0.036), 6-fold (p = 3 × 10−7) and 3-fold (p = 8 × 10−4) lower in the COVID-19 group than in the non-COVID sepsis group, and the latter two were also lower in the COVID-19 group than in stimulated healthy controls. In conclusion, it was found that a comparatively lower anticoagulant APC response in COVID-19 patients as compared to non-COVID sepsis patients, potentially linked to endothelial dysfunction, contributes to the prothrombotic phenotype of COVID-19 sepsis. |
DOI: | doi:10.3390/biomedicines12091982 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.3390/biomedicines12091982 |
| Volltext: https://www.mdpi.com/2227-9059/12/9/1982 |
| DOI: https://doi.org/10.3390/biomedicines12091982 |
Datenträger: | Online-Ressource |
Sprache: | eng |
Sach-SW: | COVID-19 |
| protein C |
| sepsis |
| thrombin |
| thrombophilia |
K10plus-PPN: | 1906636311 |
Verknüpfungen: | → Zeitschrift |
Decreased protein C pathway activity in COVID-19 compared to non-COVID sepsis / Rühl, Heiko [VerfasserIn]; 2 September 2024 (Online-Ressource)