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| Verfasst von: | Stilgenbauer, Stephan [VerfasserIn]  |
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| | Leupolt, Elke [VerfasserIn] |
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| | Ohl, Sybille [VerfasserIn] |
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| | Weiß, Gunther [VerfasserIn] |
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| | Schröder, Martin [VerfasserIn] |
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| | Döhner, Konstanze [VerfasserIn] |
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| | Bentz, Martin [VerfasserIn] |
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| | Lichter, Peter [VerfasserIn] |
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| | Döhner, Hartmut [VerfasserIn] |
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| Titel: | Heterogeneity of deletions involving RB-1 and the D13S25 locus in B-cell chronic lymphocytic leukemia revealed by fluorescence in situ hybridization1 |
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| Verf.angabe: | Stephan Stilgenbauer, Elke Leupolt, Sybille Ohl, Gunther Weiß, Martin Schröder, Konstanze Fischer, Martin Bentz, Peter Lichter, Hartmut Döhner |
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| E-Jahr: | 1995 |
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| Jahr: | August 01 1995 |
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| Umfang: | 3 S. |
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| Fussnoten: | Gesehen am 07.11.2024 |
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| Titel Quelle: | Enthalten in: Cancer research |
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| Ort Quelle: | Philadelphia, Pa. : AACR, 1916 |
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| Jahr Quelle: | 1995 |
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| Band/Heft Quelle: | 55(1995), 16, Seite 3475-3477 |
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| ISSN Quelle: | 1538-7445 |
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| Abstract: | Recently, the D13S25 locus, which is in close proximity to the retinoblastoma gene (RB-1) on chromosome band 13q14, was discussed to play a role in the pathogenesis of B-CLL. In the present study, we isolated two overlapping genomic DNA clones (termed c13S25) containing the D13S25 DNA segment and used them as probes to analyze 85 B-CLL cases by fluorescence in situ hybridization; of the 55 cases with two RB-1 copies, 13 exhibited hemizygous (n = 7) or homozygous (n = 6) deletion of D13S25. Of 29 cases with hemizygous deletion of RB-1, all but two also showed loss of D13S25 (hemizygous, n = 25; homozygous, n = 2). One case had a homozygous deletion of both loci. We conclude that deletion of D13S25 occurs in a substantial number of B-CLL without deletion of RB-1. However, in some cases there is deletion of RB-1 without loss of D13S25, suggesting that D13S25 is not the locus of the putative tumor suppressor gene. According to our data, such a gene is most likely located within the genomic region between D13S25 and RB-1. |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 1907923764 |
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| Verknüpfungen: | → Zeitschrift |
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Heterogeneity of deletions involving RB-1 and the D13S25 locus in B-cell chronic lymphocytic leukemia revealed by fluorescence in situ hybridization1 / Stilgenbauer, Stephan [VerfasserIn]; August 01 1995 (Online-Ressource)
