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| Verfasst von: | Semerci Sevimli, Tuğba [VerfasserIn]  |
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| | Ghorbani, Aynaz [VerfasserIn] |
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| | Gakhiyeva, Fidan [VerfasserIn] |
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| | Ebrahimi, Aliakbar [VerfasserIn] |
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| | Ghorbanpoor, Hamed [VerfasserIn] |
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| | Altuğ, Burcugül [VerfasserIn] |
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| | Ergen, Fulya Buge [VerfasserIn] |
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| | Ahmadova, Zarifa [VerfasserIn] |
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| | Soykan, Merve Nur [VerfasserIn] |
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| | Tufekcioglu, Emre [VerfasserIn] |
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| Titel: | Evaluation of boric acid treatment on microRNA‐127‐5p and metastasis genes orchestration of breast cancer stem cells |
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| Verf.angabe: | Tuğba Semerci Sevimli, Aynaz Ghorbani, Fidan Gakhiyeva, Aliakbar Ebrahimi, Hamed Ghorbanpoor, Burcugül Altuğ, Fulya Buge Ergen, Zarifa Ahmadova, Merve Nur Soykan, Emre Tufekcioglu |
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| Jahr: | 2024 |
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| Umfang: | 10 S. |
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| Illustrationen: | Illustrationen |
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| Fussnoten: | Online veröffentlicht: 04. Juli 2024 ; Gesehen am 19.11.2024 |
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| Titel Quelle: | Enthalten in: Biological trace element research |
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| Ort Quelle: | [Erscheinungsort nicht ermittelbar] : Springer US, 1979 |
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| Jahr Quelle: | 2024 |
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| Band/Heft Quelle: | (2024) |
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| ISSN Quelle: | 1559-0720 |
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| Abstract: | Coregulation of microRNAs (miRNAs) and cancer stem cells (CSCs) is very important in carcinogenesis. miR-127-5p is known to be downregulated in breast cancer. In this study, we aimed to investigate how boric acid (BA), known for its previously unstudied anti-cancer properties, would affect the expression of miR127-5p and genes responsible for breast cancer stem cells (BC-SCs) metastasis. BC-SCs were isolated from human breast cancer cells (MCF-7) by immunomagnetic cell separation and characterized with flow cytometry and sphere formation. The viability of BC-SCs and the determination of its IC50 value in response to boric acid (BA) were assessed via the MTT assay. Boric acid exhibited dose- and time-dependent inhibition of cell viability in cells. The IC50 doses of boric acid in MCF-7 cells and BC-SCs were 45.69 mM and 41.27 mM, respectively. The impact of BA on the expression of metastatic genes and miR127-5p was elucidated through RT-qPCR analysis. While the expression of the COL1A1 (p < 0.05) and VIM (p < 0.01) was downregulated, the expression of the miR-127-5p, ZEB1 (p < 0.01), CDH1 (p < 0.05), ITGB1 (p < 0.05), ITGA5 (p < 0.05), LAMA5 (p < 0.01), and SNAIL (p < 0.05), was up-regulated in dose-treated BC-SCs (p < 0.001) to the RT-qPCR results. Our findings suggest that boric acid could induce miR-127-5p expression. However, it cannot be said that it improves the metastasis properties of breast cancer stem cells. |
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| DOI: | doi:10.1007/s12011-024-04274-6 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext: https://doi.org/10.1007/s12011-024-04274-6 |
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| | kostenfrei: Volltext: https://link.springer.com/article/10.1007/s12011-024-04274-6#citeas |
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| | DOI: https://doi.org/10.1007/s12011-024-04274-6 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Boric acid |
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| | Breast cancer |
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| | Cancer stem cell |
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| | Metastasis |
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| | miR-127-5p |
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| K10plus-PPN: | 1908936169 |
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| Verknüpfungen: | → Zeitschrift |
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Evaluation of boric acid treatment on microRNA‐127‐5p and metastasis genes orchestration of breast cancer stem cells / Semerci Sevimli, Tuğba [VerfasserIn]; 2024 (Online-Ressource)
