| |  Online-Ressource |
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| Verfasst von: | Bouman, Brigitte J. [VerfasserIn]  |
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| | Demerdash, Yasmin [VerfasserIn] |
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| | Sood, Shubhankar [VerfasserIn] |
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| | Grünschläger, Florian [VerfasserIn] |
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| | Pilz, Franziska [VerfasserIn] |
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| | Itani, Abdul Rahman [VerfasserIn] |
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| | Kuck, Andrea [VerfasserIn] |
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| | Marot-Lassauzaie, Valérie [VerfasserIn] |
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| | Haas, Simon [VerfasserIn] |
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| | Haghverdi, Laleh [VerfasserIn] |
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| | Essers, Marieke [VerfasserIn] |
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| Titel: | Single-cell time series analysis reveals the dynamics of HSPC response to inflammation |
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| Verf.angabe: | Brigitte J. Bouman, Yasmin Demerdash, Shubhankar Sood, Florian Grünschläger, Franziska Pilz, Abdul R Itani, Andrea Kuck, Valérie Marot-Lassauzaie, Simon Haas, Laleh Haghverdi, Marieke AG Essers |
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| E-Jahr: | 2024 |
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| Jahr: | March 2024 |
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| Umfang: | 17 S. |
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| Illustrationen: | Illustrationen |
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| Fussnoten: | Gesehen am 03.12.2024 ; Online veröffentlicht: 18. Dezember 2023 |
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| Titel Quelle: | Enthalten in: Life science alliance |
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| Ort Quelle: | Heidelberg : EMBO Press, 2018 |
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| Jahr Quelle: | 2024 |
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| Band/Heft Quelle: | 7(2024), 3 vom: März, Artikel-ID e202302309, Seite 1-17 |
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| ISSN Quelle: | 2575-1077 |
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| Abstract: | Hematopoietic stem and progenitor cells (HSPCs) are known to respond to acute inflammation; however, little is understood about the dynamics and heterogeneity of these stress responses in HSPCs. Here, we performed single-cell sequencing during the sensing, response, and recovery phases of the inflammatory response of HSPCs to treatment (a total of 10,046 cells from four time points spanning the first 72 h of response) with the proinflammatory cytokine IFN alpha to investigate the HSPCs' dynamic changes during acute inflammation. We developed the essential novel computational approaches to process and analyze the resulting single-cell time series dataset. This includes an unbiased cell type annotation and abundance analysis post inflammation, tools for identification of global and cell type-specific responding genes, and a semi-supervised linear regression approach for response pseudotime reconstruction. We discovered a variety of different gene responses of the HSPCs to the treatment. Interestingly, we were able to associate a global reduced myeloid differentiation program and a locally enhanced pyroptosis activity with reduced myeloid progenitor and differentiated cells after IFN alpha treatment. Altogether, the single-cell time series analyses have allowed us to unbiasedly study the heterogeneous and dynamic impact of IFN alpha on the HSPCs. |
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| DOI: | doi:10.26508/lsa.202302309 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.26508/lsa.202302309 |
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| | Volltext: https://www.life-science-alliance.org/content/7/3/e202302309 |
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| | DOI: https://doi.org/10.26508/lsa.202302309 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | DIFFERENTIATION |
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| | EXPOSURE |
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| | HEMATOPOIETIC STEM-CELLS |
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| | METABOLIC-REGULATION |
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| K10plus-PPN: | 1910733490 |
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| Verknüpfungen: | → Zeitschrift |
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Single-cell time series analysis reveals the dynamics of HSPC response to inflammation / Bouman, Brigitte J. [VerfasserIn]; March 2024 (Online-Ressource)
