Navigation überspringen
Universitätsbibliothek Heidelberg
Status: Bibliographieeintrag

Verfügbarkeit
Standort: ---
Exemplare: ---
heiBIB
 Online-Ressource
Verfasst von:Suzuki, Risa [VerfasserIn]   i
 Woo, Jia Zheng [VerfasserIn]   i
 Thumberger, Thomas [VerfasserIn]   i
 Hofmann, Gero [VerfasserIn]   i
 Wittbrodt, Joachim [VerfasserIn]   i
 Tavhelidse-Suck, Tinatini [VerfasserIn]   i
Titel:Characterizing medaka visual features using a high-throughput optomotor response assay
Verf.angabe:Risa Suzuki, Jia Zheng Woo, Thomas Thumberger, Gero Hofmann, Joachim Wittbrodt, Tinatini Tavhelidse-Suck
E-Jahr:2024
Jahr:June 28, 2024
Umfang:14 S.
Illustrationen:Illustrationen
Fussnoten:Gesehen am 09.12.2024
Titel Quelle:Enthalten in: PLOS ONE
Ort Quelle:San Francisco, California, US : PLOS, 2006
Jahr Quelle:2024
Band/Heft Quelle:19(2024), 6, Artikel-ID e0302092, Seite 1-14
ISSN Quelle:1932-6203
Abstract:Medaka fish (Oryzias latipes) is a powerful model to study genetics underlying the developmental and functional traits of the vertebrate visual system. We established a simple and high-throughput optomotor response (OMR) assay utilizing medaka larvae to study visual functions including visual acuity and contrast sensitivity. Our assay presents multiple adjustable stripes in motion to individual fish in a linear arena. For that the OMR assay employs a tablet display and the Fish Stripes software to adjust speed, width, color, and contrast of the stripes. Our results demonstrated that optomotor responses were robustly induced by black and white stripes presented from below in the linear-pool-arena. We detected robust strain specific differences in the OMR when comparing long established medaka inbred strains. We observed an interesting training effect upon the initial exposure of larvae to thick stripes, which allowed them to better respond to narrower stripes. The OMR setup and protocol presented here provide an efficient tool for quantitative phenotype mapping, addressing visual acuity, trainability of cortical neurons, color sensitivity, locomotor response, retinal regeneration and others. Our open-source setup presented here provides a crucial prerequisite for ultimately addressing the genetic basis of those processes.
DOI:doi:10.1371/journal.pone.0302092
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

kostenfrei: Volltext: https://doi.org/10.1371/journal.pone.0302092
 kostenfrei: Volltext: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0302092
 DOI: https://doi.org/10.1371/journal.pone.0302092
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Animal behavior
 Cameras
 Inbred strains
 Larvae
 Swimming
 Vertebrates
 Vision
 Visual acuity
K10plus-PPN:1911213504
Verknüpfungen:→ Zeitschrift

Permanenter Link auf diesen Titel (bookmarkfähig):  https://katalog.ub.uni-heidelberg.de/titel/69281679   QR-Code
zum Seitenanfang