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Verfasst von:Thaden, Ole [VerfasserIn]   i
 Schneider, Nicole [VerfasserIn]   i
 Walther, Tobias [VerfasserIn]   i
 Spiller, Erin [VerfasserIn]   i
 Taoum, Alexandre [VerfasserIn]   i
 Göpfrich, Kerstin [VerfasserIn]   i
 Duarte Campos, Daniela Filipa [VerfasserIn]   i
Titel:Bioprinting of synthetic cell-like lipid vesicles to augment the functionality of tissues after manufacturing
Verf.angabe:Ole Thaden, Nicole Schneider, Tobias Walther, Erin Spiller, Alexandre Taoum, Kerstin Göpfrich, and Daniela Duarte Campos
E-Jahr:2024
Jahr:July 18, 2024
Umfang:11 S.
Fussnoten:Gesehen am 09.12.2024
Titel Quelle:Enthalten in: American Chemical SocietyACS synthetic biology
Ort Quelle:Washington, DC : ACS, 2012
Jahr Quelle:2024
Band/Heft Quelle:13(2024), 8, Seite 2436-2446
ISSN Quelle:2161-5063
Abstract:Bioprinting is an automated bioassembly method that enables the formation of human tissue-like constructs to restore or replace damaged tissues. Regardless of the employed bioprinting method, cells undergo mechanical stress that can impact their survival and function postprinting. In this study, we investigate the use of a synthetic cell-like unit, giant unilamellar vesicles (GUVs), as adjuvants of the cellular function of human cells postprinting, or in future as the complete replacement of human cells. We analyzed the impact of two nozzle-based bioprinting methods (drop-on-demand and extrusion bioprinting) on the structure, stability, and function of GUVs. We showed that over 65% of the GUVs remain intact when printing at 0.5 bar, demonstrating the potential of using GUVs as a synthetic cell source. We further increased the stability of GUVs in a cell culture medium by introducing polyethylene glycol (PEG) into the GUV lipid membrane. The presence of PEG, however, diminished the structural properties of GUVs postprinting, and reduced the interaction of GUVs with human cells. Although the design of PEG-GUVs can still be modified in future studies for better cell-GUV interactions, we demonstrated that GUVs are functional postprinting. Chlorin e6-PEG-GUVs loaded with a fluorescent dye were bioprinted, and they released the dye postprinting only upon illumination. This is a new strategy to deliver carriers, such as growth factors, drugs, nutrients, or gases, inside large bioprinted specimens on a millimeter to centimeter scale. Overall, we showed that printed GUVs can augment the functionality of manufactured human tissues.
DOI:doi:10.1021/acssynbio.4c00137
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

kostenfrei: Volltext: https://doi.org/10.1021/acssynbio.4c00137
 DOI: https://doi.org/10.1021/acssynbio.4c00137
Datenträger:Online-Ressource
Sprache:eng
K10plus-PPN:1911499335
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