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Status: Bibliographieeintrag

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Verfasst von:Yamamoto, Akihisa [VerfasserIn]   i
 Sakamaki, Yusuke [VerfasserIn]   i
 Abuillan, Wasim [VerfasserIn]   i
 Konovalov, Oleg [VerfasserIn]   i
 Ueno, Yuko [VerfasserIn]   i
 Tanaka, Motomu [VerfasserIn]   i
Titel:Structural and mechanical characterization of DNA-tethered membranes on graphene
Verf.angabe:Akihisa Yamamoto, Yusuke Sakamaki, Wasim Abuillan, Oleg Konovalov, Yuko Ueno, and Motomu Tanaka
E-Jahr:2024
Jahr:July 24, 2024
Umfang:8 S.
Fussnoten:Gesehen am 16.12.2024
Titel Quelle:Enthalten in: Langmuir
Ort Quelle:Washington, DC : ACS Publ., 1985
Jahr Quelle:2024
Band/Heft Quelle:40(2024), 31, Seite 16530-16537
ISSN Quelle:1520-5827
Abstract:Lipid membranes that are separated from the surface of graphene by DNA tethers were prepared by surface functionalization with pyrene coupled to single-stranded DNA (ssDNA), followed by self-assembly of the mixture of ssDNA-functionalized phospholipid and the matrix phospholipids. The formation of uniform membranes was confirmed by fluorescence microscopy, and the structures of the systems before and after hybridization in the direction perpendicular to the global plane of the membranes were investigated using high-energy X-ray reflectivity. The thickness values of the DNA spacers (15 and 37 bp) calculated from the best-fit results were less than the expected thicknesses of the double-stranded DNA (dsDNA) chains taking the upright conformation, indicating that the DNA spacers are tilted with respect to the direction normal to the surface. The Young’s moduli of the DNA-tethered membranes obtained by AFM nanoindentation showed higher values than the membranes with no DNA tethers, which suggests that the DNA layer resists against the compression, lifting up the membrane. Intriguingly, the presence of DNA tethers caused no increase in the yield depth. The smaller thickness values as well as the unchanged yield depth suggest that the dsDNA chains can tilt and rotate, which can be attributed to the flexible pyrene-DNA junction.
DOI:doi:10.1021/acs.langmuir.4c01957
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

kostenfrei: Volltext: https://doi.org/10.1021/acs.langmuir.4c01957
 DOI: https://doi.org/10.1021/acs.langmuir.4c01957
Datenträger:Online-Ressource
Sprache:eng
K10plus-PPN:1912380013
Verknüpfungen:→ Zeitschrift

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