Effects of fixation on the preservation of peroxisomal structures for immunofluorescence studies using HepG2 cells as a model system

• Verfasst von: Schrader, Michael [VerfasserIn]
• Verfasst von: Baumgart-Vogt, Eveline [VerfasserIn]
• Verfasst von: Fahimi, H. Dariush [VerfasserIn]
• Titel: Effects of fixation on the preservation of peroxisomal structures for immunofluorescence studies using HepG2 cells as a model system
• Verf.angabe: Michael Schrader, Eveline Baumgart, H. Dariush Fahimi
• E-Jahr: 1995
• Jahr: August 1995
• Umfang: 5 S.
• Fussnoten: Gesehen am 14.01.2025
• Titel Quelle: Enthalten in: The histochemical journal
• Ort Quelle: Dordrecht [u.a.] : Springer Science + Business Media B.V., 1968
• Jahr Quelle: 1995
• Band/Heft Quelle: 27(1995), 8, Seite 615-619
• ISSN Quelle: 1573-6865
• DOI: doi:10.1007/BF00173099
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Cell Culture
• Sach-SW: Fixation Procedure
• Sach-SW: HepG2 Cell
• Sach-SW: Special Importance
• Sach-SW: Spherical Particle
• K10plus-PPN: 1914570456

Abstract

The immunofluorescence technique has become an important tool for the investigation of peroxisomes in cell culture. We have used this method for the study of peroxisomes in the human hepatoblastoma cell line HepG2. A marked heterogeneity of peroxisomal forms was detected. Besides spherical (about 100 nm) and rod-shaped structures (about 300 nm) many elongated, undulating tubular forms (up to 5 μm) were found. Further observations indicate that the appearance of the peroxisomal forms in immunofluorescence is dependent on the fixation procedure used. Whereas the fixation with methanol-acetone (−20°C) or ethanol results in a punctate pattern with spherical particles, the use of formaldehyde/Triton X-100 fixation shows well-preserved tubules and rods. These observations may be of special importance for studies on the biogenesis of peroxisomes.