Investigation of peroxisomal lipid beta-oxidation enzymes in guinea pig liver peroxisomes by immunoblotting and immunocytochemistry

• Verfasst von: Yamamoto, K. [VerfasserIn]
• Verfasst von: Völkl, Alfred [VerfasserIn]
• Verfasst von: Fahimi, H. Dariush [VerfasserIn]
• Titel: Investigation of peroxisomal lipid beta-oxidation enzymes in guinea pig liver peroxisomes by immunoblotting and immunocytochemistry
• Verf.angabe: Kouta Yamamoto, Alfred Völkl, H. Dariush Fahimi
• E-Jahr: 1992
• Jahr: December 1992
• Umfang: 10 S.
• Fussnoten: Gesehen am 15.01.2025
• Titel Quelle: Enthalten in: Journal of histochemistry & cytochemistry
• Ort Quelle: London [u.a.] : Sage Publ., 1953
• Jahr Quelle: 1992
• Band/Heft Quelle: 40(1992), 12, Seite 1909-1918
• ISSN Quelle: 1551-5044
• DOI: doi:10.1177/40.12.1360481
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 191461500X

Abstract

We investigated the immunoreactivity of the peroxisomal lipid beta-oxidation enzymes acyl-CoA oxidase, trifunctional protein, and thiolase in guinea pig liver and compared it with that of homologous proteins in rat, using immunoblotting of highly purified peroxisomal fractions and monospecific antibodies to rat proteins. In addition, the immunocytochemical localization of beta-oxidation enzymes in guinea pig liver was compared with that of catalase. All antibodies showed crossreactivity between the two species, indicating that these peroxisomal proteins have been well conserved, although all exhibited some differences with respect to molecular size and, in the case of acyl-CoA oxidase, in frequency of the immunoreactive bands. In the latter case, a distinct second band in the 70 KD range was observed in guinea pig, in addition to the regular band due to subunit A present in rat liver. This novel band could be due either to trihydroxycoprostanoyl-CoA oxidase or to the non-inducible branched chain fatty acid oxidase described recently. All three beta-oxidation enzymes were immunolocalized by light and electron microscopy to the matrix of peroxisomes, in contrast to catalase, which is also found in the cytoplasm and the nucleus of hepatocytes in guinea pig liver.