In vitro chondrogenic induction promotes the expression level of IL-10 via the TGF-β/SMAD and canonical Wnt/β-catenin signaling pathways in exosomes secreted by human adipose tissue-derived mesenchymal stem cells

• Verfasst von: Semerci Sevimli, Tuğba [VerfasserIn]
• Verfasst von: Inan, Ulukan [VerfasserIn]
• Verfasst von: Mantar, Dilara [VerfasserIn]
• Verfasst von: Guler, Kubra [VerfasserIn]
• Verfasst von: Ahmadova, Zarifa [VerfasserIn]
• Verfasst von: Gulec, Kadri [VerfasserIn]
• Verfasst von: Topal, Ahmet Emin [VerfasserIn]
• Titel: In vitro chondrogenic induction promotes the expression level of IL-10 via the TGF-β/SMAD and canonical Wnt/β-catenin signaling pathways in exosomes secreted by human adipose tissue-derived mesenchymal stem cells
• Verf.angabe: Tugba Semerci Sevimli, Ulukan Inan, Dilara Mantar, Kubra Guler, Zarifa Ahmadova, Kadri Gulec, Ahmet Emin Topal
• E-Jahr: 2024
• Jahr: December 2024
• Umfang: 10 S.
• Illustrationen: Illustrationen
• Fussnoten: Gesehen am 27.01.2025
• Titel Quelle: Enthalten in: Cell biochemistry and biophysics
• Ort Quelle: New York, NY : Springer, 1995
• Jahr Quelle: 2024
• Band/Heft Quelle: 82(2024), 4 vom: Dez., Seite 3741-3750
• ISSN Quelle: 1559-0283
• DOI: doi:10.1007/s12013-024-01461-z
• Datenträger: Online-Ressource
• Sprache: eng
• Bibliogr. Hinweis: Errata: Semerci Sevimli, Tuğba: Correction: In vitro chondrogenic induction promotes the expression level of IL-10 via the TGF-β/SMAD and canonical Wnt/β-catenin signaling pathways in exosomes secreted by human adipose tissue-derived mesenchymal stem cells
• Bibliogr. Hinweis: Errata: Semerci Sevimli, Tuğba: Correction: in vitro chondrogenic induction promotes the expression level of IL-10 via the TGF-β/SMAD and canonical Wnt/β-catenin signaling pathways in exosomes secreted by human adipose tissue-derived mesenchymal stem cells
• K10plus-PPN: 1915631068

Abstract

Current treatment approaches cannot exactly regenerate cartilage tissue. Regarding some problems encountered with cell therapy, exosomes are advantageous because of their “cell-free” nature. This study examines the relationship between IL-10 and TGF-β and Canonical Wnt/β-catenin signal pathways in human adipose tissue-derived MSCs exosomes (hAT-MSCs-Exos) after in vitro chondrogenic differentiation. Human adipose tissue-derived mesenchymal stem cells (hAT-MSCs) and, as a control group, human fetal chondroblast cells (hfCCs) were differentiated chondrogenically in vitro. Exosome isolation and characterization analyses were performed. Chondrogenic differentiation was shown by Alcian Blue and Safranin O stainings. The expression levels of IL-10, TGF-β/SMAD signaling pathway genes, and Canonical Wnt/β-catenin signaling pathway genes, which play an essential role in chondrogenesis, were analyzed by RT-qPCR. Conditioned media cytokine levels were measured by using the TGF-β and IL-10 ELISA kits. IL-10 expression was upregulated in both chondrogenic differentiated hAT-MSC-Exos (dhAT-MSC-Exos) (p < 0.0001). In the TGF-β signaling pathway, TGF-β (p < 0.0001), SMAD2 (p < 0.0001), SMAD4 (p < 0.001), ACAN (p < 0.0001), SOX9 (p < 0.05) and COL1A2 (p < 0.0001) expressions were upregulated in dhAT-MSC-Exos. SMAD3 expression was upregulated in non-differentiated hAT-MSC-Exos. In the Canonical Wnt/β-catenin signaling pathway, WNT (p < 0.0001) and CTNNB1(p < 0.0001) expressions were upregulated in dhAT-MSC-Exos. AXIN (p < 0.0001) expression was upregulated in non-differentiated hAT-MSC-Exos. TGF-β and IL-10 levels were higher in dhAT-MSCs) (p < 0.0001). Related to these results, IL-10 may induce TGF-β/SMAD and Canonical Wnt/β-catenin signaling pathways in hAT-MSC exosomes obtained after chondrogenic differentiation. Therefore, using these exosomes for cartilage regeneration can lead to the development of treatment methods.