| Online-Ressource |
Verfasst von: | Sun, Quanchao [VerfasserIn]  |
| Picascia, Tiziana [VerfasserIn]  |
| Khan, Arif ul Maula [VerfasserIn]  |
| Brenna, Cinzia [VerfasserIn]  |
| Heuveline, Vincent [VerfasserIn]  |
| Schmaus, Anja [VerfasserIn]  |
| Sleeman, Jonathan P. [VerfasserIn]  |
| Gretz, Norbert [VerfasserIn]  |
Titel: | Application of ethyl cinnamate based optical tissue clearing and expansion microscopy combined with retrograde perfusion for 3D lung imaging |
Verf.angabe: | Quanchao Sun, Tiziana Picascia, Arif ul Maula Khan, Cinzia Brenna, Vincent Heuveline, Anja Schmaus, Jonathan P. Sleeman and Norbert Gretz |
E-Jahr: | 2020 |
Jahr: | 12 Oct 2020 |
Umfang: | 16 S. |
Fussnoten: | Gesehen am 27.01.2025 |
Titel Quelle: | Enthalten in: Experimental lung research |
Ort Quelle: | Abingdon : Taylor & Francis Group, 1980 |
Jahr Quelle: | 2020 |
Band/Heft Quelle: | 46(2020), 10, Seite 393-408 |
ISSN Quelle: | 1521-0499 |
Abstract: | 3 D imaging of the lung is not a trivial undertaking as during preparation the lung may collapse. Also serial sections and scans followed by 3 D reconstruction may lead to artifacts. The present study aims to figure out the best way to perform 3 D imaging in lung research. We applied an optical tissue clearing (OTC) method, which uses ethyl cinnamate (ECi) as a fast, nontoxic and cheap clearing solvent, for 3 D imaging of retrograde perfused lungs by laser confocal fluorescence microscopy and light sheet fluorescence microscopy. We also introduced expansion microscopy (ExM), a cutting-edge technique, in 3 D imaging of lungs. We examined and compared the usefulness of these techniques for 3 D lung imaging. The ExM protocol was further extended to paraffin-embedded lung metastases blocks. The MHI148-PEI labeled lung vasculature was visualized by retrograde perfusion combined with trachea ligation and ECi based OTC. As compared with trans-cardiac perfusion, the retrograde perfusion results in a better maintenance of lung morphology. 3 D structure of alveoli, vascular branches and cilia in lung were revealed by immunofluorescence staining after ExM. 3 D distribution of microvasculature and neutrophil cells in 10 years old paraffin-embedded lung metastases were analyzed by ExM. The retrograde perfusion combined with trachea ligation technique could be applied in the lung research in mice. 3 D structure of lung vasculature can be visualized by MHI148-PEI perfusion and ECi based OTC in an efficient way. ExM and immunofluorescence staining protocol is highly recommended to perform 3 D imaging of fresh fixed lung as well as paraffin-embedded lung blocks. |
DOI: | doi:10.1080/01902148.2020.1829183 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1080/01902148.2020.1829183 |
| Volltext: https://www.tandfonline.com/doi/full/10.1080/01902148.2020.1829183 |
| DOI: https://doi.org/10.1080/01902148.2020.1829183 |
Datenträger: | Online-Ressource |
Sprache: | eng |
Sach-SW: | Ethyl cinnamate |
| expansion microscopy |
| lung |
| retrograde perfusion |
| three-dimensional imaging |
K10plus-PPN: | 191567896X |
Verknüpfungen: | → Zeitschrift |
Application of ethyl cinnamate based optical tissue clearing and expansion microscopy combined with retrograde perfusion for 3D lung imaging / Sun, Quanchao [VerfasserIn]; 12 Oct 2020 (Online-Ressource)