Increased messenger Rna levels for low-density lipoprotein receptor and 3-hydroxy-3-methylglutaryl coenzyme A reductase in rat liver after long-term ethanol ingestion

• Verfasst von: Seitz, Helmut K. [VerfasserIn]
• Verfasst von: Kuhn, Bernhard [VerfasserIn]
• Verfasst von: Hodenberg, Eberhard von [VerfasserIn]
• Verfasst von: Fiehn, Walter [VerfasserIn]
• Verfasst von: Conradt, Christian [VerfasserIn]
• Verfasst von: Simanowski, Ulrich [VerfasserIn]
• Titel: Increased messenger Rna levels for low-density lipoprotein receptor and 3-hydroxy-3-methylglutaryl coenzyme A reductase in rat liver after long-term ethanol ingestion
• Verf.angabe: Helmut K. Seitz, Bernhard Kuhn, Eberhard von Hodenberg, Walter Fiehn, Christian Conradt, Ulrich A. Simanowski
• Jahr: 1994
• Umfang: 7 S.
• Fussnoten: Gesehen am 03.02.2025
• Titel Quelle: Enthalten in: Hepatology
• Ort Quelle: [Alphen aan den Rijn] : Wolters Kluwer Health, 1981
• Jahr Quelle: 1994
• Band/Heft Quelle: 20(1994), 2, Seite 487-493
• ISSN Quelle: 1527-3350
• DOI: doi:10.1002/hep.1840200230
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1916188435

Abstract

Because long-term alcohol intake leads to severe alterations of cholesterol metabolism resulting in both elevated serum cholesterol levels and increased hepatic concentrations of cholesterol esters, we investigated the effect of long-term ethanol consumption on the hepatic messenger RNA (mRNA) content of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase and low-density lipoprotein receptor, two major regulatory factors in cholesterol metabolism, and of apoprotein E. Twenty-four male Sprague-Dawley rats were pair-fed nutritionally adequate liquid diets containing 36% of total calories as either ethanol or isocaloric carbohydrates for 3 wk. In addition, the lipid content of the diets was varied, resulting in 35%, 17.5%, and 8.8% of total calories corresponding to a daily intake of cholesterol of between 1.2 and 6.3 mg/kg body wt. Although increasing dietary cholesterol intake resulted in a significant decrease of hepatic mRNA for low-density lipoprotein receptor and HMG-CoA reductase (p<0.05), long-term ethanol consumption led to a significant increase of the mRNAfor both proteins(p<0.01), and this increase was predominantly obvious in animals fed a low-cholesterol diet. In contrast, mRNA content of apoprotein E was found to be significantly lower in livers from rats fed ethanol for a prolonged period of time as compared with controls (p<0.01), and this effect was found to be still present, although less pronounced, after low cholesterol intake. These findings were paralleled by a significant increase in hepatic cholesterol esters (161 ± 30 vs. 70 ± 25 mg/100 gm; p<0.01) but not free cholesterol and by elevated serum cholesterol levels (129 ± 8 vs. 85 ± 10 mg/100 ml; p<0.01). The data show that long-term ethanol consumption results in enhanced hepatic mRNA levels of low-density lipoprotein receptor and HMG-CoA reductase, two determinants of cholesterol metabolism, which may contribute to hepatic cholesterol accumulation and hypercholesterolemia frequently seen in the alcoholic. (Hepatology 1994;20:487-493.)