| Online-Ressource |
Verfasst von: | Eble, Michael J. [VerfasserIn]  |
| Hensley, Frank W. [VerfasserIn]  |
| Flentje, Michael [VerfasserIn]  |
| Schlotz, Annette [VerfasserIn]  |
| Wannenmacher, Michael [VerfasserIn]  |
Titel: | A modified computer-assisted colorimetric microtitre assay (MTT) to assess in vitro radiosensitivity of V79, CaSki, HeLa and WiDr cells |
Verf.angabe: | M.J. Eble, F.W. Hensley, M. Flentje, A. Schlotz, M. Wannenmacher |
Jahr: | 1994 |
Umfang: | 9 S. |
Fussnoten: | Gesehen am 06.02.2025 ; Elektronische Reproduktion der Druck-Ausgabe 3. Juli 2009 |
Titel Quelle: | Enthalten in: International journal of radiation biology |
Ort Quelle: | London : Taylor & Francis, 1959 |
Jahr Quelle: | 1994 |
Band/Heft Quelle: | 65(1994), 2, Seite 193-201 |
ISSN Quelle: | 1362-3095 |
Abstract: | The non-clonogenic MTT assay, based on the reduction of a tetrazolium salt to a purple formazan precipitate by living cells, was modified and a new procedure of analysis is proposed. The colorimetric assay could be performed semiautomatically using microtitre plate reader connected to a personal computer. Data are processed and plotted with a customized program. To ensure that both control and irradiated microtitre plates contain exponentially growing cells at the time of analysis, the calculation of relative survival is based on a series of well-defined cell numbers initially seeded. To make the two endpoints of non-clonogenic and clonogenic assays comparable, i.e. counting of living cells versus counting of colonies, radiation-induced progression delay was incorporated into the calculation. Radiation-induced cell killing and progression delay could be determined in a single analysis, but in an independent way. X-ray survival curves were generated for V79, CaSki, WiDr and HeLa cells using the non-clonogenic and a standard clonogenic assay. Using the linear-quadratic formula, the resulting parameters α,β and the mean inactivation dose were not significantly different. The described assay is a feasible and reproducible technique for determination of cellular survival, which may be able to incorporate progression delay. The equivalence to a clonogenic survival assay could be proven. |
DOI: | doi:10.1080/09553009414550231 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1080/09553009414550231 |
| DOI: https://doi.org/10.1080/09553009414550231 |
Datenträger: | Online-Ressource |
Sprache: | eng |
K10plus-PPN: | 1916564690 |
Verknüpfungen: | → Zeitschrift |
¬A¬ modified computer-assisted colorimetric microtitre assay (MTT) to assess in vitro radiosensitivity of V79, CaSki, HeLa and WiDr cells / Eble, Michael J. [VerfasserIn]; 1994 (Online-Ressource)