Navigation überspringen
Universitätsbibliothek Heidelberg
Status: Bibliographieeintrag

Verfügbarkeit
Standort: ---
Exemplare: ---
heiBIB
 Online-Ressource
Verfasst von:Werle, Egon [VerfasserIn]   i
 Seitz, Gisela [VerfasserIn]   i
 Kohl, Brigitte [VerfasserIn]   i
 Fiehn, Walter [VerfasserIn]   i
 Seitz, Helmut K. [VerfasserIn]   i
Titel:High-performance liquid chromatopgraphy improves diagnostic efficiency of carbohydrate-deficient transferrin
Verf.angabe:Egon Werle, Gisela E. Seitz, Brigitte Kohl, Walter Fiehn and Helmut K. Seitz
E-Jahr:1997
Jahr:01 January 1997
Umfang:7 S.
Fussnoten:Gesehen am 06.02.2025
Titel Quelle:Enthalten in: Alcohol and alcoholism
Ort Quelle:Oxford : Oxford Univ. Press, 1963
Jahr Quelle:1997
Band/Heft Quelle:32(1997), 1, Seite 71-77
ISSN Quelle:1464-3502
Abstract:Carbohydrate-deficient transferrin (CDT) is considered a useful biochemical marker of regular high alcohol intake. CDT was measured in the sera of 51 alcohol abusers, 20 patients with nonalcoholic liver disease and 30 healthy controls with an alcohol intake of <30g/day. The mean CDT levels of these three groups respectively were determined with high-performance liquid chromatography (HPLC; 4.6 ± 5.2%; 0.7 ± 0.2%; 0.7 ± 0.2%) and with a radioimmunoassay after microcolumn anion-exchange chromatography (MAEC/RIA; 34.2 ± 26.9 U/1; 16.9 ± 3.8 U/1; 18.0 ± 5.7 U/1). CDT levels in patients with severe alcohol abuse (161.6 ± 96.4g/day) were significantly higher than in the two other groups under investigation (P < 0.0001). In heavily drinking subjects, the mean daily alcohol intake correlated with aspartate aminotransferase levels (ASAT) but not with the CDT levels determined either with HPLC or MAEC/RIA. With both methods, the CDT levels were slightly higher in patients with an ASAT concentration >30 U/1, which may indicate an advanced liver damage (P < 0.05). Analysis of receiver-operating characteristic (ROC) plots demonstrated that the diagnostic accuracy of the HPLC method, which determines the relative amount of CDT, was significantly higher than the established MAEC/RIA method, which measures the absolute amount of CDT (area under the ROC curve: 0.95 ± 0.02 vs 0.73 ± 0.05; P < 0.0001). At a specificity of >95%, the sensitivity of CDT determined with HPLC and MAEC/RIA was 80 and 47%, respectively. In addition, HPLC may be a useful and reliable method for the determination of this important biochemical marker, since the HPLC chromatogram is a visible document of the successful isotransferrin separation and measurement.
DOI:doi:10.1093/oxfordjournals.alcalc.a008236
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.1093/oxfordjournals.alcalc.a008236
 DOI: https://doi.org/10.1093/oxfordjournals.alcalc.a008236
Datenträger:Online-Ressource
Sprache:eng
K10plus-PPN:1916589081
Verknüpfungen:→ Zeitschrift

Permanenter Link auf diesen Titel (bookmarkfähig):  https://katalog.ub.uni-heidelberg.de/titel/69301641   QR-Code
zum Seitenanfang