Effects of oxiris® therapy on cytokine elimination after a LPS infusion

• Verfasst von: Kalenka, Armin [VerfasserIn]
• Verfasst von: Arens, Philipp [VerfasserIn]
• Verfasst von: Müllenbach, Ralf M. [VerfasserIn]
• Verfasst von: Weigand, Markus A. [VerfasserIn]
• Verfasst von: Brune, Maik [VerfasserIn]
• Verfasst von: Fiedler-Kalenka, Mascha [VerfasserIn]
• Titel: Effects of oxiris® therapy on cytokine elimination after a LPS infusion
• Titelzusatz: an experimental animal study
• Verf.angabe: Armin Kalenka, Philipp Arens, Ralf M. Müllenbach, Markus A. Weigand, Maik Brune and Mascha O. Fiedler-Kalenka
• E-Jahr: 2024
• Jahr: 27 August 2024
• Umfang: 13 S.
• Illustrationen: Illustrationen
• Fussnoten: Im Text ist "®" hochgestellt ; Gesehen am 10.02.2025
• Titel Quelle: Enthalten in: International journal of molecular sciences
• Ort Quelle: Basel : Molecular Diversity Preservation International, 2000
• Jahr Quelle: 2024
• Band/Heft Quelle: 25(2024), 17, Artikel-ID 9283, Seite 1-13
• ISSN Quelle: 1422-0067
• ISSN Quelle: 1661-6596
• DOI: doi:10.3390/ijms25179283
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: hemadsorption
• Sach-SW: inflammation
• Sach-SW: interleukin
• Sach-SW: lipopolysaccharide
• Sach-SW: sepsis
• K10plus-PPN: 1916740901

Abstract

The clinical effectiveness of Oxiris®, particularly in reducing cytokines, remains uncertain due to the limited data provided. This study explored and analyzed the application value of Oxiris® endotoxin adsorption technology in a large animal model. Pigs received an intravenous LPS infusion. Six animals were treated 2 h after the infusion with an Oxiris® hemadsorption using a pumpless extracorporeal technique for 6 h. Five animals served as controls. Cardiocirculatory parameters, hyperspectral analysis, and a panel of cytokines were measured. The lipopolysaccharide infusion induced sepsis-like inflammation with tachycardia, elevated pulmonary pressure, elevated lactate level, as well as elevated pro-inflammatory cytokines like interferon (IFN)-γ, interleukin (IL)-1β, IL-2, IL-6, IL-8, IL-12 and tumor necrosis factor alpha (TNF-α). In addition, increases of anti-inflammatory cytokines like IL-1ra and IL-10 were found. After 3 and 6 h in both groups, pro-inflammatory cytokines were significantly reduced. No differences between the intervention and the control group could be detected after 3 and 6 h for IL-1β, IL-2, IL-6, IL-8, IL-12 and TNF-α, suggesting no effect of the Oxiris® filter on the elimination of elevated cytokines with a pumpless extracorporeal hemadsorption technique. The presented large animal model may be a promising option for studying the effects of hemadsorption techniques.